| Annexin B1:一种新的细胞凋亡检测用蛋白 |
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| 引用本文： | 张毅,郭瀛军,王芳,高远舰,颜宏利,孙树汉.Annexin B1:一种新的细胞凋亡检测用蛋白[J].第二军医大学学报,2003,24(3):333-334. |
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| 作者姓名： | 张毅 郭瀛军 王芳 高远舰 颜宏利 孙树汉 |
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| 作者单位： | 第二军医大学基础医学部医学遗传学教研室,上海,200433 |
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| 基金项目： | 国家“8 6 3”计划项目 (2 0 0 1AA2 13111) |
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| 摘 要： | 目的：为细胞凋亡研究提供一种新的检测用蛋白。方法：在大肠杆菌中表达Annexin B1蛋白，经离子交换层析后得纯品，异硫氰酸荧光黄（fluorescein isothiocyanate,FITC)标记Annexin B1；选用Jarkat细胞，羟基喜树碱诱导该细胞凋亡；以FITC标记的Annexin B1检测凋亡细胞。结果：FITC标记的Annexin B1与凋亡细胞膜表面磷脂酰丝氨酸（phos-phatidylserine,PS)结合，使凋亡细胞呈阳性着色，能有效检测细胞凋亡。结论：开发得到了一种新的细胞凋亡检测用蛋白Annextin B1，为推广高效快捷的凋亡检测技术提供国产试剂奠定了基础。
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| 关 键 词： | AnnexinB1 细胞凋亡 检测用蛋白 |
| 文章编号： | 0258-879X(2003)03-0333-02 |
| 修稿时间： | 2003年1月6日 |
Annexin B1 as a novel protein for detecting apoptosis |
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| ZHANG Yi,GUO Ying Jun,WANG Fang,GAO Yuan Jian,YAN Hong Li,SUN Shu Han.Annexin B1 as a novel protein for detecting apoptosis[J].Academic Journal of Second Military Medical University,2003,24(3):333-334. |
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| Authors: | ZHANG Yi GUO Ying Jun WANG Fang GAO Yuan Jian YAN Hong Li SUN Shu Han |
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| Institution: | ZHANG Yi,GUO Ying Jun,WANG Fang,GAO Yuan Jian,YAN Hong Li,SUN Shu Han * |
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| Abstract: | Objective: To provide a novel protein for detecting apoptosis. Methods: Annexin B1 was expressed in E. coli and purified with ion exchange chromatography. Then Annexin B1 labelled with FITC was used to detect the apoptosis of Jurkat cells,which was induced by camptothecin. Results: The FITC labelled Annexin B1 efficiently detected apoptosis with positive fluorescent signal when it bound to phosphatidylserine on apoptotic cell membrane. Conclusion: Annexin B1 may be a novel protein which can be utilized in detecting apoptosis. |
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| Keywords: | Annexin B1 apoptosis |
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