| 阳离子PLGA载基因纳米粒的初步研究 |
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| 引用本文： | 李洪英,邹伟伟,刘春喜,张娜. 阳离子PLGA载基因纳米粒的初步研究[J]. 中国生化药物杂志, 2009, 30(2) |
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| 作者姓名： | 李洪英 邹伟伟 刘春喜 张娜 |
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| 作者单位： | 山东大学,药学院,药物制剂研究所,山东,济南,250012 |
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| 摘 要： | 目的制备并评价阳离子PLGA载基因纳米粒(pDNA-CTAB-NPs)。方法纳米沉淀法制备空白阳离子PLGA纳米粒(CTAB-NPs),与报告基因pEGFP复合,考察其理化性质、抗核酸酶降解能力、体外释药特性、细胞毒性及其在A549细胞中的转染情况。结果pDNA-CTAB-NPs呈球形或类球形,平均粒径175.5 nm,Zeta电位+12.54mV,pDNA结合充分(>95%),抗核酸酶降解能力强,具有缓释效果,符合Higuchi释放动力学方程(r=0.997 7),细胞毒性较低,能在A549细胞中表达。结论pDNA-CTAB-NPs是一种制备工艺简单,性能良好,极富潜力的非病毒纳米基因载体。
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| 关 键 词： | 阳离子PLGA纳米粒 CTAB 非病毒基因载体 |
Preliminary studies on pDNA loaded cationic PLGA nanoparticles |
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| LI Hong-ying,ZOU Wei-wei,LIU Chun-xi,ZHANG Na. Preliminary studies on pDNA loaded cationic PLGA nanoparticles[J]. Chinese Journal of Biochemical Pharmaceutics, 2009, 30(2) |
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| Authors: | LI Hong-ying ZOU Wei-wei LIU Chun-xi ZHANG Na |
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| Affiliation: | Institute of Pharmaceutics;School of Pharmaceutical Science;Shandong University;Jinan 250012;China |
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| Abstract: | Purpose To prepare and to investigate the pDNA loaded cationic PLGA nanoparticles(pDNA-CTAB-NPs).Methods Blank cationic PLGA nanoparticles(CTAB-NPs)were prepared by nanoprecipitation method,and the report gene pEGFP was incubated with the CTAB-NPs at room temperature leading to the formation of the pDNA-CTAB-NPs.The physico-chemical properties,protection of the pDNA-CTAB-NPs to pDNA from nuclease degradation,release behaviour,in vitro cytotoxicity as well as transfection activity of pDNA-CTAB-NPs were evalu... |
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| Keywords: | CTAB |
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