阿霉素诱导人卵巢癌细胞凋亡和周期特异性的实验研究

目的 :探讨阿霉素诱导卵巢癌细胞凋亡的规律。方法 :以不同浓度的阿霉素作用于人卵巢癌细胞株SKOV3,用光镜、电镜、TUNEL法和DNA凝胶电泳法检测确定细胞凋亡 ,以流式细胞仪定量分析凋亡和细胞周期特异性。结果 :大剂量 ( 2 5～10 0 μg/ml)长时间 ( 3～ 72h)的阿霉素作用可引起卵巢癌细胞坏死和凋亡 ,较小剂量( 0 .5μg/ml)的阿霉素连续作用 2 4、4 8、72h ,诱导细胞凋亡发生率分别为 4 0 .0 1%、65.38%和 77.65% ,并使细胞发生G1期阻滞。结论 :阿霉素能导致卵巢癌细胞DNA损伤 ,发生G1期阻滞并诱导凋亡是其抗肿瘤的机制之一。

Experimental study of doxorubicin inducing apoptosis in human ovarian carcinoma cell line

Objective： To explore the effect of apoptosis induced by various concentration of doxorubicin at different time in ovarian carcinoma cell line SKOV3 in vitro. Methods： Apoptosis were evaluated by light and electron microscopy. DNA agarol gel electrophoresis, terminal deoxynucleotidyl transferase mediated dUTP in situ nick end labeling. The quantitative of apoptosis and cell's cycle specificity anaysis were measured by flow cytometry. Results： Ovarian carcinoma cells were treated with higher concentration doxorabicin(25-100μg/ml)at 3～72 h could induce necrosis and apoptosis. The lower concentration doxorubicin(0.5μg/ml)could induce mainly ovarian carcinoma cells apoptosis in G1 phase. Conclusions： Doxorubicin can damage ovarian carcinoma cell's DNA,induct the cells G1 phase arrest and induce cells apoptosis. It is one of the mechanism of anti-cancer chemotherapy of doxorubicin.