黄米醇溶蛋白对小鼠胆固醇代谢的调节作用

比较了分别饲喂酪蛋白、大豆分离蛋白、黄米醇溶蛋白小鼠的血清总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇,甘油三酯浓度和动脉硬化指数,旨在评价黄米醇溶蛋白对小鼠胆固醇代谢的特定作用。结果显示:黄米醇溶蛋白(添加1.82%Lys和0.23%Trp)能显著提高小鼠血清高密度脂蛋白胆固醇浓度(P<0.05),降低动脉硬化指数;但体外实验表明:黄米醇溶蛋白不能抑制羟甲基戊二酸单酰辅酶A(HMG-CoA)还原酶的活性,即不能抑制胆固醇的合成。线性回归分析得出黄米醇溶蛋白对小鼠血清胆固醇代谢的调节作用与其蛋氧酸与甘氨酸含量之比、疏水性氨基酸、含硫氨基酸含量密切相关。

Effects of Proso Millet Prolamin on Plasma Cholesterol Metabolism in Mice

Present study aims to evaluate the effect of proso millet prolamin(PMP) on cholesterol(metabolism) in mice,by comparing the concentration of plasma total (cholesterol),high-density lipoprotein cholesterol(HDL-c) with triglyceride of mice given the diets containing casein,soy protein isolated or(PMP(supplemented) with 1.82%Lys and 0.23%Trp).The mice fed with PMP diet showed the highest(HDL-c) content significantly(P<0.05) and the lowest atherogenic index among three groups.In vitro,(PMP couldn't) inhibit the activity of 3-hydroxy-3-methylglutaryl-coenayme A(HMG-CoA) reductase,and the synthesis of cholesterol directly.On the basis of the linear regression analysis,the content of sulfur-containing amino acids,hydrophobic amino acids,the ratio of Met to Gly(Methionine/Glycine) of PMP were important factors in modulating the metabolism of serum cholesterol in mice.