鼠雾化吸入环丙沙星后血药浓度的影响

目的研究鼠雾化吸入环丙沙星后血液中环丙沙星的浓度。方法制作气管插管铜绿假单胞菌生物膜感染模型,用生理盐水（第1组）,红霉素（第2组）,环丙沙星（第3组）和红霉素联合环丙沙星（第4组）雾化干预模型大鼠7天,采用高效液相色谱法测定鼠血浆中环丙沙星的浓度。结果标准曲线回归方程为：y=311.01x-11.333R=0.9999,其线性范围为0.1～10μg/ml。血浆中环丙沙星定量下限为0.1μg/ml。高效液相色谱法测环丙沙星日间和日内精密度RSD均小于10%,体内回收率RSD小于5%,与血浆杂质达到基线分离R〉1.5。各样本血浆中环丙沙星浓度均低于环丙沙星标准曲线中所能测定的环丙沙星的最低值0.1ug/ml。结论使用高效液相色谱法测定血浆环丙沙星灵敏、专一、重复性好。雾化方式吸入的环丙沙星几乎不通过局部组织进入血液循环。

Effect of drug concentration in blood plasma after inhaling ciprofloxacin in rats model

Objective To investigate concentration of ciprofloxacin in blood after using inhalation of ciprofloxacin in rats model, Methods Rats model of Pseudomonas aeruginosa biofilm infection has been established.A 7 days of intervention with the follow 4 groups：control group（groupl）,erythromycin alone（group2）,ciprofloxacin alone（group3） and combination of both（group4） had been done. Concentration of ciprofloxacin in rats model＇s blood plasma was measured by High Performance Liquid Chromatography （HPLC）. Results The standard curve regression equation was y=311.01x-11.333R=0.9999.The linear range was 0.1- 10 μg/ml.The low limit of ciprofloxacin＇s quantitation was 0.1 μg/ml.All of the with day＇s and the between day＇s degree of precision （RSD） of ciprofloxacin by HPLC were less than 10%.The recovery rates （RSD） in vivo were less than 5%. The foreign substance in blood plasma could been separated at the baseline （R〉 1.5）. Concentration of ciprofloxacin in samples＇ blood plasma was less than 0. 1μg/ml which was the standard curve＇ minimum value. Conclusions It was sensitive and good in reproducibility that using HPLC measured ciprofloxacin＇s concentration. Ciprofloxacin by inhalation almost didn＇t enter the blood circulation by across part tissues.