| 甲状腺激素对成骨样MG-63细胞甲状腺激素受体不同亚型表达的影响 |
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| 引用本文： | 杨宇虹,左爱军,朱志峰,赵学勤,郭刚,张镜宇.甲状腺激素对成骨样MG-63细胞甲状腺激素受体不同亚型表达的影响[J].中华内分泌代谢杂志,2011,27(3). |
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| 作者姓名： | 杨宇虹 左爱军 朱志峰 赵学勤 郭刚 张镜宇 |
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| 作者单位： | 1. 天津医科大学基础医学院生化教研室,300070
2. 天津医科大学代谢病医院内分泌研究所卫生部激素与发育重点实验室 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 检测人类成骨样细胞MG-63中甲状腺激素受体(TR)的表达情况以及甲状腺激素(T3或T4)对其的影响.方法 用实时荧光定量PCR法对MG-63细胞中TR的主要4个亚型,α1、α1、β1、β2的mRNA表达进行了测定.结果 TRα1 mRNA的表达水平最高,为10.70±0.45;TRβ1仅及其半,为5.75±0.10;TRβ2不低,相当于TRβ1的60%,为3.34±0.08;TRα2的表达极低,仅为(3.66±0.59)×10-2.分别加入不同浓度的T3或T4,仅TRα1和TRα2对T3表现明显的降调节,且TRα1和TRα2的表达与相应T3浓度之间负相关,其他亚型无显著性变化,也无相关关系;同样浓度范围的T4作用各亚型,表达均无显著性变化.结论 在骨细胞发育分化中TRα1起关键作用.Abstract：Objective To examine the expression of thyroid hormone receptor isoforms (TR α1, α2, β1, and β2) in human osteoblast-like cell line MG-63 at the mRNA level and the effect of thyroid hormone (T3 or T4 ) on the expression. Methods Realtime quantitative PCR was performed. Results The expression of TRα1 mRNA was the highest, that was 10. 70± 0.45, TRβ1 was 5.75 ± 0. 10, TRβ2 was 3.34 ± 0. 08, and TRα2 was very low, only (3.66 ±0. 59) × 10-2. Only the expression of TRαl and TRα2 mRNA was down regulated significantly by the treatment of 10-10 ～ 10-6 mol/L T3, and there was a negative correlation between the expression of TRα1 or TRα2 mRNA and the concentration of T3. Conclusion TRα1 plays a primary role in mediating the effects of thyroid hormones in skeletal development.
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| 关 键 词： | 甲状腺激素 甲状腺激素受体 人类成骨样细胞系MG-63 基因表达 |
Effect of thyroid hormones on the expression of thyroid hormone receptor isoforms in human osteogenic sarcoma cell line MG-63 |
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| YANG Yu-hong,ZUO Ai-jun,ZHU Zhi-feng,ZHAO Xue-qin,GUO Gang,ZHANG Jing-yu.Effect of thyroid hormones on the expression of thyroid hormone receptor isoforms in human osteogenic sarcoma cell line MG-63[J].Chinese Journal of Endocrinology and Metabolism,2011,27(3). |
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| Authors: | YANG Yu-hong ZUO Ai-jun ZHU Zhi-feng ZHAO Xue-qin GUO Gang ZHANG Jing-yu |
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| Abstract: | Objective To examine the expression of thyroid hormone receptor isoforms (TR α1, α2, β1, and β2) in human osteoblast-like cell line MG-63 at the mRNA level and the effect of thyroid hormone (T3 or T4 ) on the expression. Methods Realtime quantitative PCR was performed. Results The expression of TRα1 mRNA was the highest, that was 10. 70± 0.45, TRβ1 was 5.75 ± 0. 10, TRβ2 was 3.34 ± 0. 08, and TRα2 was very low, only (3.66 ±0. 59) × 10-2. Only the expression of TRαl and TRα2 mRNA was down regulated significantly by the treatment of 10-10 ～ 10-6 mol/L T3, and there was a negative correlation between the expression of TRα1 or TRα2 mRNA and the concentration of T3. Conclusion TRα1 plays a primary role in mediating the effects of thyroid hormones in skeletal development. |
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| Keywords: | Thyroid hormones Thyroid hormone receptors Human osteoblast-like cell line MG-63 Gene expression |
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