| 巴戟天不同农家类型种质资源的RAPD分析 |
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| 引用本文： | 丁平,徐吉银,楚桐丽. 巴戟天不同农家类型种质资源的RAPD分析[J]. 中药材, 2006, 29(1): 1-3 |
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| 作者姓名： | 丁平 徐吉银 楚桐丽 |
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| 作者单位： | 广州中医药大学中药学院,广东,广州,510405 |
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| 基金项目： | 广东省博士启动基金;广东省科技厅科技计划 |
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| 摘 要： | 目的:探讨不同农家类型巴戟天在分子水平上的遗传多样性。方法:利用分子生物学技术中随机扩增多态性DNA(RAPD)技术对巴戟天5个不同农家类型的种质资源进行检测。结论:40个随机引物中有14个引物扩增产物具多态性,每个多态性引物平均可扩增出3~5个片段。聚类分析表明,全部材料可分为两大类,其中大叶种聚为一类,小叶种单独为一类。结果:不同农家类型巴戟天种质资源在分子水平上存在明显的遗传差异。
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| 关 键 词： | 巴戟天 遗传多样性 DNA指纹图谱 |
| 文章编号： | 1001-4454(2006)01-0001-03 |
| 收稿时间： | 2005-05-10 |
| 修稿时间： | 2005-10-12 |
RAPD Analysis on Germplasm Resources of Different Farm Races of Morinda officinalis |
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| DING Ping,XU Ji-yin,CHU Tong-li. RAPD Analysis on Germplasm Resources of Different Farm Races of Morinda officinalis[J]. Journal of Chinese medicinal materials, 2006, 29(1): 1-3 |
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| Authors: | DING Ping XU Ji-yin CHU Tong-li |
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| Affiliation: | College of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China |
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| Abstract: | Objective: To explore the genetic diversity of different farm races of Morinda officinalis on molecular level.Methods: The molecular biological technique-random amplified polymorphic DNA(RAPD) were used.Results: Of the 40-mer arbitary primers,14 were found to amplify polymorphic products.3-5 polymorphic bands were amplified by each polymorphic primer on the average.Using UPGMA method all the tested accesions can be clustered into two groups: 4 accessions of Daye were classified as one group,1 accession of Xiaoye be another group.Conclusion: There actually existed much genetic diversity on molecular level among the different farm races of Morinda officinalis. |
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| Keywords: | RAPD |
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