大川芎丸对大鼠脑缺血的保护作用

目的　研究大川芎丸对大鼠脑缺血的保护作用。方法　采用大鼠大脑中动脉缺血 (MCAO)模型 ,将2 0只 SD大鼠随机分成治疗组和对照组 ,每组各 10只 ,分别于术后每天予以大川芎丸提取物 4 .13g/kg或相应剂量生理盐水 ,72 h后参照 L onga法行神经功能评分后处死 ,取脑组织作石蜡切片行 HE染色 ,计算脑体梗死积比。结果　大川芎丸治疗组神经功能评分 (1.5± 0 .71)低于对照组 (2 .3± 0 .82 ) ,差异有显著性 (P=0 .0 32 )。大川芎丸治疗组梗死体积比 (10 .81± 1.6 0 )与对照组 (17.82± 1.4 8)相比较 ,脑梗塞体积比明显减少 ,差异有显著性 (P=0 .0 0 0 )。结论　中药大川芎丸对大鼠脑缺血有保护作用。

The Protective Effect of Dachuanxiongwan on the Rats with Focal Cerebral Ischemia and Reperfusion

OBJECTIVE: Studying the protective effect of dachuanxiongwan (DCXW) on the rats with focal cerebral ischemia and reperfusion. METHODS: The ischemia and reperfusion model was established by blocking the rat's middle cerebral artery with a pre-set nylon thread and removing it from the internal carotid artery 2 hours after occlusion. 20 healthy male Sprague-Dawley rats following middle cerebral artery occlusion (MCAO) were randomly divided into two groups: Group A, transient ischemia, for control; Group B, transient ischemia treated with DCXW via gavage, 4.13 g/kg x d, twice a day for 3 days, while group A being given the same volume of saline. Seventy-two hours after reperfusion, the neurological function of all rats was evaluated according to Longa, and the rats were sacrificed. All the samples of cerebrum were processed and embedded in paraffin and cut into sections. The sections were stained with Hematoxylin & Eosin to measure the infarction volume and evaluate the effects of DCXW. RESULTS: The grade of neurological function of the treatment group (1.5+/-0.71) was lower than that of the control group (2.3+/-0.82), there was a statistically significant difference between the two groups (P=0.032); the infarction volume of the treatment group was smaller than that of the control group, there was a statistically significant difference between the two groups (P=0.000). CONCLUSION: The findings suggest that DCXW has a protective effect on the cerebrum undergoing ischemia and reperfusion.