| 细胞表面分子在间充质干细胞对T细胞调节机制中的作用 |
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| 引用本文： | 洪再发,熊共鹏,张建溪.细胞表面分子在间充质干细胞对T细胞调节机制中的作用[J].医疗保健器具,2011(2):170-172. |
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| 作者姓名： | 洪再发 熊共鹏 张建溪 |
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| 作者单位： | 厦门中医院肝外科; |
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| 基金项目： | 国家卫生部联合攻关基金(WKJ2005-2-020) |
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| 摘 要： | 目的探索细胞表面免疫分子在间充质干细胞（mesenchymal stem cells，MSC）对T细胞的免疫调节机制中的作用。方法分离、培养和鉴定MSC和T细胞；电镜观察MSC和T细胞共同培养时的相互作用；检测MSC对T细胞表面分子CD54，程序性死亡受体（programmed cell death ligand，PDL）-1，组织相容性复合物（major histocompatibility complex，MHC）-I，MHC—II表达的影响。结果MSC与T细胞共培养时，大量MSC和T细胞连接在一起：T细胞表面分子的表达率：离体实验一买验组：CD54：（66．0±1．3）％；PDL-1：（61．0±19）％，MHC－I：（23．4±3．4）％，MHC－II：（27．5±1．2）％；对照组：CD54：（52．1±2．0）％，PDL-1：（42．1±2．0）％，MHC－I：（19．5±3．0）％，MHC－II：（9．2±0．8）％；在体实验一实验组：CD54：（59．0±2．6）％；PDL－l：（57．4±2．4）％；MHC－I：（22．6±2．7）％；MHC-II：（22．4±2．0）％；对照组：CD54：（51．5±1．0）％，PDL-1：（50．0±3．3）％，MHC－I：（18．1±1．5）％，MHC－II：（9．7±0．6）％。结论MSC与T细胞相互接触后促进T细胞表达CD54，PDL-1，MHc－I，MHC－II，这可能在MSC对T细胞免疫调节作用中发挥重要作用。
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| 关 键 词： | MSC T细胞 免疫调节 机制 细胞因子 |
The Role of Cell Surface Molecules in the Regulatory Mechanism of MSC on T-cell |
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| HONG Zaifa,XIONG Gongpeng,ZHANG Jianxi.The Role of Cell Surface Molecules in the Regulatory Mechanism of MSC on T-cell[J].Medicine Healthcare Apparatus,2011(2):170-172. |
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| Authors: | HONG Zaifa XIONG Gongpeng ZHANG Jianxi |
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| Institution: | HONG Zaifa,XIONG Gongpeng,ZHANG Jianxi(Department of Hepatobiliary Surgery,Xiamen Traditional Hospital,Xiamen 361004,China) |
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| Abstract: | Objective To explore the role of cell surface immune molecules in the regulatory mechanism of MSC on T-cell.Methods MSC and T cell were isolated,cultured and identified.The electron microscopy was used to observe interaction between MSC and T cell.The expression rates of CD54,PDL-1,MHC-I,MHC-II on T cell in vitro and in vivo were detected with flow cytometer.Results T cell contacted with MSC when they were cultured together.The expression rate of surface molecule on T cell in vitro-experimental group:CD54:(... |
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| Keywords: | MSC T cell Immunological regulation Mechanism Cytokine |
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