参附注射液对大鼠小肠缺血-再灌注后肾损伤的影响

目的 观察参附注射液(SF)对大鼠小肠缺血-再灌注后肾结构、功能和肾组织中bcl-2、bax表达水平的影响. 方法将36只SD大鼠随机平分为假手术组 (C组),缺血-再灌注＋0.9%氯化钠溶液组(IR＋NS组),缺血-再灌注＋参附注射液预处理组(IR＋SF组),各12只. IR＋NS组和IR＋SF组夹闭大鼠肠系膜上动脉60 min后松开建立小肠缺血-再灌注模型,C组穿线但不结扎. C组和IR＋NS组分别于阻断前30 min用微量泵经股静脉恒速泵入0.9%氯化钠溶液10 mL•kg^-1,IR＋SF组同法泵入参附注射液10 mL•kg^-1. 光镜下观察肾组织结构,i-STAT自动分析仪检测血清尿素(Urea)及电解质含量,免疫组化方法检测肾组织中bcl-2、bax蛋白的表达,TUNEL法检测肾脏细胞的凋亡水平. 结果 光镜下可见IR＋NS组肾组织结构损伤较IR＋SF组严重. IR＋NS组血清Urea和 钾离子(K＋)水平分别为(15.88±1.38 )和(6.88±0.43)mmol•L^-1, C组分别为(5.82±0.39)和(4.28±0.24 ) mmol•L^-1,IR＋SF组分别为(8.03±0.77)和(5.03±0.31 )mmol•L^-1,IR＋NS组和IR＋SF组与C组比较,差异有统计学意义(P＜0.01),IR＋SF组较IR＋NS组显著降低(P＜0.01). IR＋NS组bcl-2和bax阳性细胞率分别为(0.242 5±0.036 5)%和(0.281 7±0.029 2)%,显著高于C组分别为(0.013 7±0.008 6)%和(0.011 7± 0.009 4)%](P＜0.01), IR＋SF组分别为(0.176 3±0.030 2)%和(0.196 7±0.024 6)%,较IR＋NS组显著降低(P＜0.01). C组凋亡指数(apoptotic index,AI)为(0.014 2±0.009 0)%,IR＋NS组为(0.179 2±0.028 4)%,IR＋SF组为(0.124 2±0.026 4)%,IR＋NS组和IR＋SF组与C组比较,差异有统计学意义(P＜0.01),IR＋SF组较IR＋NS组显著降低(P＜0.01). 结论 参附注射液对小肠缺血-再灌注造成的肾损伤组织具有保护作用.

Effect of Shenfu Injection on Renal Injury after Intestinal Ischemic Reperfusion in Rats

Objective To investigate the effect of shenfu injection(SF) on the kidney structure and function,and expression of bcl-2 and bax in kidney after intestinal ischemic reperfusion. Methods 36 SD rats were randomly and equally divided into 3 groups:group C,as control;group IR+NS,as ischemic reperfusion and NS pretreatment;group IR+SF,ischemic reperfusion and SF pretreatment.The model of intestinal ischemic reperfusion was established by blocking the superior mesenteric artery for 60 minutes in both IR+NS and IR...