Mindray BC-5300自动血细胞分析仪性能的初步评价

目的:评价BC-5300血细胞分析仪全血细胞计数(CBC)和白细胞分类(DC)的性能。方法:按照有关文件规定对该仪器CBC的性能指标进行评价,并与人工分类进行比较。结果:该仪器CBC的精密度、携带污染率和总重复性均符合要求,在正常及常见的病理范围线性良好,与XE-2100的结果比较,相关性好(r2>0.994);DC:中性粒细胞(Neut)、淋巴细胞(Lym)、单核细胞(Mon)、嗜酸性粒细胞(Eos)和嗜碱性粒细胞(Baso)的重复性好。WBC形态正常的样本,仪器与人工DC的结果比较,Neut、Lym和Eos相关性较好(r=0.9663,r=0.9595,r=0.9178);Mon次之(r=0.9104),Baso的相关性欠佳(r=0.5065)。一定比例的幼稚细胞和异常淋巴细胞可出现WBC散点图变化或报警,提示人工涂片镜检。结论:该仪器检测的结果准确、可靠,主要性能指标符合实验要求;对怀疑WBC分类异常的标本,仪器DC结果必须经显微镜复核后才能报告。

Performance evaluation of mindray BC-5300 hematology analyzer

Objective： To evaluate the performance of Mindray BC-5300 hematology analyzer on completing blood cell counting（CBC） and leukocyte differential count（DC）. Methods： The indicators of CBC performance were evaluated by following guidelines and compared with manual operation. Results： The precision, carry-over rate, and total repeatability meet the standards, and the linearity was also reliable both in physiogenic and pathological conditions. Comparing with the results from XE-2100, the correlation was also satisfied（r2 0.994）. It has better repeatability in neutrophilic granulocytes, lymphocytes, eosinophile granulocyte, and basophile granulocyte. The WBC samples with morphological performance were compared between instrumental and manual DC operation. The repeatability in neutrophilic granulocytes, lymphocytes and eosinophile granulocyte（r=0.9663, r=0.9595, r=0.9178）, the r value of monocyte was 0.9104 and basophil was 0.5065. The detection of juvenile cell and abnormal lymphocyte suggested manual review. Conclusion： The results from BC-5300 automatic hematology analyzer were accurate and reliable, the main indicators could all meet the clinical needs. The suggestive diagnosis by leukocyte scatterplot and abnormal leukocytes classification should be double checked by manual smear microscopic examination.