载脂蛋白H基因多态性与冠心病关系的研究

目的探讨载脂蛋白H(ApoH)外显子3、8基因多态性与冠心病(CHD)的关系。方法采用聚合酶链式反应结合限制性片段长度多态分析方法,分析了100例健康人及110例冠心病患者的ApoH外显子3、8基因型。结果CHD组外显3GG基因型的频率为81.8%,GA+AA基因型频率为11.8%,G等位基因频率为88%,A等位基因频率是12%,与对照组比较无差异,CHD组外显子8GG基因型频率为74.5%、GC基因型频率为25.5%,G等位基因频率为87%,C等位基因频率为13%,与对照组比较CHD组的GC基因型频率及C等位基因频率显著增高。结论ApoH外显子3基因多态性与CHD无相关性;8GC基因型及C等位基因与CHD有关。

Relationship between apolipoprotein H Gene polymorphism and coronary heart disease

Objective To investigate relationship between the polymorphism of apolipoprotein H(ApoH) exon3 and exon 8 polymorphismand coronary heart disease(CHD).Methods Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of ApoH exon 3 and exon8 in 110 patients with CHD and 100 health control group. Results The genotype GG of ApoH exon 3 in the CHD group was 81.8%,GA AA genotype was only 11.8%,the frequency of G allele was 88%,while 12%in the A allele;there was no significant difference with the control groups The frequency of GG genotype in ApoH exon 8 were 74.5%,GC genotype were25.5%,the frequency of G allele was 87%;the frequency of GC genotype and C allele in CHD group were significant higher than that in control group.LDL level in CHD group was significant higher than that in control group(P<0.05).Conclusion The polymorphism of the ApoH exon3 have no relationship with CHD but the GC genotyp of ApoH exon3 and C allele have close relation with CHD and serum TG level.