京尼平交联改性可溶性蛋壳膜蛋白冻干支架的制备及检测

目的：评价京尼平交联改性前后可溶性蛋壳膜蛋白（soluble eggshell membrane protein,SEP）冻干组织工程支架的理化性能和生物相容性。方法：冷冻干燥法制备SEP的冻干支架,浸泡于京尼平溶液中交联改性。通过扫描电镜观察其表面形貌。测量其孔隙率、抗拉强度以及降解率,采用溶血实验、亚急性全身毒性实验和细胞毒性实验初步评价其生物相容性。结果：京尼平交联改性前后的SEP冻干支架孔径分别为（280±71）μm和（263±89）μm,孔隙率分别为（90.4±7.6）%和（87.9±9.7）%;交联改性显著提高了SEP冻干支架的拉伸强度和弹性模量,降低了支架的失重率（P〈0.01）;交联前后的材料均无溶血现象;亚急性短期全身毒性实验中组织切片均未见病理变化;细胞毒性检测均为0级。结论：京尼平交联改性在提高SEP冻干支架的力学强度和抗降解能力的同时,仍可保持支架材料良好的生物相容性。

Preparation and evaluation of a freeze-dried genepin-crosslinked SEP scaffold

Objective:To evaluate the physicochemical properties and biocompatibility of a freeze-dried soluble eggshell membrane protein(SEP) scaffold crosslinked by genipin.Method:A porous SEP scaffold was prepared by freeze-drying and then crosslinked with genipin.Surface morphology and internal structure of the scaffolds were observed by scanning electron microscope(SEM).The porosity,tensile strength and degradation rate were measured.The hemolysis test,subacute systematic toxicity test and cytotoxicity test were p...