| 三维培养的牙髓干细胞参与牙髓损伤修复能力的实验研究 |
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| 引用本文： | 王亦菁,张晓东,李里,金岩,史俊南.三维培养的牙髓干细胞参与牙髓损伤修复能力的实验研究[J].临床口腔医学杂志,2011,27(10):582-584. |
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| 作者姓名： | 王亦菁 张晓东 李里 金岩 史俊南 |
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| 作者单位： | 1. 沈阳军区总医院口腔内科,辽宁,沈阳,110840
2. 第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的：将体外建立的细胞因子BMP-2作用下牙髓干细胞（DPSC）三维培养体系应用于大鼠牙髓损伤模型中,观察DPSC对牙髓组织损伤修复的能力。方法：已构建的三维培养DPSC的细胞团添加BMP-2体外持续培养7 d,BrdU标记及检测鉴定后,将其置于建立的大鼠牙髓损伤模型洞内,移植4周后HE染色,改良Mallory三色法染色观察,免疫组化检测BrdU标记的细胞分布。结果：移植后4周,DPSC三维细胞培养的牙髓盖髓实验中有骨样牙本质基质形成,没有观察到组织的炎症和坏死。在基质中可以看到骨性成牙本质样细胞,带有长的突起的成牙本质样细胞在骨性牙本质中形成管状牙本质。结论：损伤的牙髓表面植入添加BMP处理的DPSC细胞团能够产生修复性牙本质。
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| 关 键 词： | 牙髓干细胞 三维培养 牙髓损伤模型 |
Effect of dental pulp stem cell cultured by three dimensions on reparation of dental injury |
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| WANG Yi-jing,ZHANG Xiao-dong,LI Li,JIN Yan,SHI Jun-nan.Effect of dental pulp stem cell cultured by three dimensions on reparation of dental injury[J].Journal of Clinical Stomatology,2011,27(10):582-584. |
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| Authors: | WANG Yi-jing ZHANG Xiao-dong LI Li JIN Yan SHI Jun-nan |
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| Institution: | WANG Yi-jing1,ZHANG Xiao-dong1,LI Li1,JIN Yan2,SHI Jun-nan2.1.The Department of Stomatology,General Hospital of Shenyang Military Command,Liaoning Shenyang 110840,China,2.The College of Stomatology,Forth Military Medical University,Shanxi Xi'an 710032 |
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| Abstract: | Objectine:In the rat dental injury model,3-dimension culture system of dental pulp stem cell(DPSC) affected by BMP-2 was used.The ability of DPSC to the dental injury was observed.Method:The DPSC mass in the 3-dimension culture system was cultured for 7 days after added with BMP-2.The cell mass was marked and identified by Brdu,then was put in the holes of rat dental injury model.Result:Osteoid dentin substance was found in the 3-dimension culture system of DPSC after 4 weeks.No tissue inflammation and necr... |
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| Keywords: | dental stem cell 3-dimension culture injured dental model |
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