HGF在人牙周膜成纤维细胞中的表达

目的：观察HGF在人牙周膜成纤维细胞中的表达及探讨转化生长因子-β1（TGF-β1）、白细胞介素-1β（IL-1β）与HGF之间的关系。方法：分别采用IL-1β梯度浓度、最佳干预浓度和TGF-β1梯度浓度干预第5代人牙周膜成纤维细胞,并运用酶联免疫技术检测人牙周膜成纤维细胞的上清液中HGF的表达。结果：经IL-1β单独作用,人牙周膜成纤维细胞的上清液中HGF的含量较空白对照组高,并优选出IL-1β的最佳干预浓度为10 ng/mL。当一定浓度的TGF-β1和10 ng/mL的IL-1β联合干预人牙周膜成纤维细胞,该细胞上清液中HGF的含量较单独IL-1β作用组低。结论：TGF-β1能够抑制IL-1β诱导的人牙周膜成纤维细胞分泌HGF。

Hepatocyte growth factor expression by regionally defined human periodontal ligament fibroblasts

Objective：Purpose of this study is to observe the expression of the hepatocyte growth factor（HGF） in human periodontal ligament fibroblasts and to explore the relationship of transforming growth factor-β1（TGF-β1）,interleukin-1β（IL-1β） and HGF.Method：Respectively the concentration gradient of IL-1β,the best markedly concentration of IL-1β and the concentration gradient of TGF-β1 interfered the cells of the 5th generation of HPLFs,then examined the expression of HGF in HPLFs by ELISA method.Result：The IL-1β alone,the HGF content in the supernatant of HPLFs more than in the blank control group,and found the best markedly concentration of IL-1β is 10 ng/mL.When a certain concentration of TGF-β1 and 10 ng/mL IL-1β joint intervention HPLFs,the HGF content in the supernatant of HPLF than separate the role of IL-1β were lower.Conclusion：TGF-β1 can inhibite the IL-1β induced HGF production in HPLFs.