快速去除冰冻红细胞甘油的方法改进研究

目的 建立冰冻红细胞快速去甘油的方法。方法 采用三步法快速去除冰冻红细胞中的甘油。通过控制制备时盐水的浓度、作用时间及离心次数,用高浓度盐水大量稀释冰冻红细胞后,离心去除盐水,加入相应体积的生理盐水重悬,来达到快速去除甘油的目的。分别对比了常规法和改良法制备的冰冻解冻去甘油红细胞的血红蛋白含量、游离血红蛋白含量、甘油残留量、白细胞残留量及血细胞比容5个方面的指标。结果 传统去甘油的方法大约需要1 h,三步法去甘油大约需要15 min,极大地缩短了时间,提高了效率。对去甘油红细胞进行相关检测,甘油残留量和白细胞残留量符合《全血与成分血质量要求(2012版)》的规定,血红蛋白含量略低于规定。结论 快速解冰冻去甘油方法具有时间短、效率高的优点,但仍需进一步改进和优化以使产品质量全面达到国家标准。

Establishing A Method to Rapidly Remove Glycerolin Frozen Red Blood Cells

Abstract:Objective To establish a method to rapidly remove glycerol in frozen red blood cells(RBC).Methods The glycerol in frozen RBC was removed by using three-step method as following:thefrozen RBC were diluted by adding salt solution with high concentration.The salt solution was removed through centrifugation.The RBC were resuspended in normal saline of corresponding volume.Three-step method could achieve the quick removal of glycerol by controlling the concentration salt water and action time.The indexes of hemoglobin,free hemoglobin,glycerol residue,residual white bloodcell(WBC)and hematocrit of frozen-deglycerolized RBC were compared between conventional and improved methods.Results The traditionalmethod to remove glycerol needed 1h approximately,while the three-step methodonly needed 15 mins approximately,greatly shortening the time and improving theefficiency.The related tests for removing glycerol RBC,except hemoglobin content was slightly lower than the “The quality requirements for whole blood and blood components(2012 edition)”,the rest of them all meeted the requirements.Conclusion The three-step method has the advantages of shorttime and high efficiency.However,it still needs to be further improved and optimized to make the quality of product meet the national standard.