CD133-2在急性白血病病程中的表达及意义

目的 探讨CD133-2在急性白血病病程中的表达及其临床意义。方法 通过直接免疫荧光流式细胞术对67例不同病程急性白血病(AL)进行CD133-2的检测。结果 AL组CD133-2的阳性率(52.4%)及表达率(23.9%±21.5%)均明显高于对照组(0,2.2%±3.9%); 初治组、完全缓解期(CR)组及复发组CD133-2的阳性率分别为52.4%,0和40.0%,表达率分别为23.9%±21.5%,5.0%±6.0%和28.4%±25.6%,三组间的阳性率及表达率差异有统计学意义(χ2=12.777,F=5.906,P<0.05)。初治组及复发组的CD133-2阳性率及表达率均明显高于CR组。初治患者CD34+组的CD133-2阳性表达率明显高于CD34-组(40.5% vs 7.1%,χ2=8.636,P<0.05),并且CD133-2-/CD34-组的CR率则明显高于CD133-2+/CD34+组(83.3% vs 33.3%,χ2=6.078,P<0.05)。结论 初治AL患者CD133表达与CD34相关; CD133/CD34共同高表达可能是AL的一个不良预后因素; 检测AL患者骨髓中CD133-2的表达可以作为预测复发及监测MRD的指标之一。

Expression of CD133-2 during the Courses of Acute Leukemia and Its Clinical Significance

Abstract:Objective To explore the expression of CD133-2during the treatment course of acute leukemia(AL)and its clinical significance.Methods Used flow cytometry with direct immunofluorescence staining to analyze CD133-2 of 67 acute leukemia patients with different treatment courese.Results The CD133-2 positive rate(52.4%)and expression rate(23.9%±21.5%)in AL were significantly higher than those in control(0,2.2%±3.9%).The CD133-2 positive rates of cases for primary treatment group,CR group and recurrence group were 52.4%,0 and 40.0% respectively,and expression rates were 23.9%±21.5%,5.0%±6.0% and 28.4%±25.6% respectively.There were significant difference in the positive rate and expression rate of CD133-2 among the three group(χ2=12.777,F=5.906,P<0.05).The CD133-2 positive rates and expression rates in primary treatment group and recurrence group were significantly higher than those in complete remission cases.CD133-2 positive rate of CD34+ group was obviously higher than that of CD34-group(40.5% vs 7.1%,χ2=8.636,P<0.05),and the CR rate of CD133-2-/CD34- group was significantly higher than that of CD133-2+/CD34+ group(83.3% vs 33.3%,χ2=6.078,P<0.05).Conclusion The expression of CD133-2 was correlated with CD34,and CD133/CD34 co-overexpression might be a bad prognostic factor of AL.CD133-2 can be used as one of the indicator of predicting recurrence and monitoring MRD.