IL-6基因启动子区域-572C>G和-174G>C多态性与江苏宿迁汉族人群冠心病的相关性

目的 探讨白细胞介素6(interleukin-6,IL-6)基因启动子区域-572C>G和-174G>C基因多态性与宿迁汉族人群冠心病(coronary heart disease,CHD)的关系。方法 参考2010卫生部CHD诊断标准(WS319-2010),选择2017年1～12月在宿迁市第一人民医院心内科确诊的CHD患者,应用聚合酶链-限制性片段长度多态性(Polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法检测266例CHD患者和192例体检健康人群(对照组),分析其IL-6-572C>G和IL-6-174G>C基因多态性,并进行Hardy-Weinberg平衡检验,检测血糖、血脂等生化指标。结果 IL-6-572C>G基因型和等位基因频率在两组间分布差异有统计学意义(χ2=11.1,19.5,22.4,均P<0.05),CHD组GG型和CG型OR值分别是CC型的4.88倍(95%CI:2.31～10.31)和1.96倍(95% CI:1.32～2.91); -174G>C基因型和等位基因频率在两组间分布差异无统计学意义(χ2=0.024,0.027,均P>0.05)。结论 IL-6-572G等位基因可能是宿迁汉族CHD的易感基因,IL-6-174G>C基因多态性可能与宿迁汉族人群CHD没有相关性。

Association between Interleukin-6-572C>G and -174G>C Gene Polymorphism in the Promoter Region and Coronary Heart Disease of Han Population in Suqian of Jiangsu

Abstract:Objective To investigate therelationship of interleukin 6(IL-6)gene promoter region upstream of the -572C>G and -174G>C gene polymorphism with coronary heart disease(CHD)of han population in Suqian ofJiangsu.Methods Reference 2010 CHD diagnostic criteria of the Ministry of Health(WS319-2010),detecting the polymorphism of IL-6-572C>G and -174G>C gene polymorphism in 266 CHD patients and 192 healthy population by polymerase chain restriction fragment length polymorphism(PCR-RFLP)and Hardy-Weinberg equilibrium was tested,measuring the concentration of blood sugar,lipid.Results At IL-6-572C>G,the OR about GG and type CG in CHD group were CC 4.88 times(95% CI:2.31～10.31)and 1.96times(95% CI:1.32～2.91)respectively.The difference of genotype and alleles werestatistical significance between the two groups(χ²=11.1,19.5 and 22.4,all P<0.05).At IL-6-174C>G,the difference of genotype and alleles were no statistical significance between the two groups(χ²=0.024 and 0.027,all P>0.05).Conclusion IL-6-572G allele may be a susceptible gene of CHD in Han population in Suqian.However IL-6-174G>C gene polymorphism is not related to CHD Han population in Suqian.