| 标准使用碳酸氢盐缓冲液进行恶性疟原虫培养和耐药性实验的研究 |
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| 引用本文： | 何深一,古钦民,李瑛,周怀瑜,丛华,赵群力. 标准使用碳酸氢盐缓冲液进行恶性疟原虫培养和耐药性实验的研究[J]. 中国病原生物学杂志, 2002, 15(1): 14-17 |
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| 作者姓名： | 何深一 古钦民 李瑛 周怀瑜 丛华 赵群力 |
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| 作者单位： | 山东大学医学院寄生虫学教研室,山东济南,250012 |
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| 摘 要： | 目的变化使用碳酸氢盐缓冲液以探讨药物对恶性疟原虫的不同效果.方法用[3H]次黄嘌呤摄取法检测抗疟药物效果.结果不同二氧化碳浓度引起的pH变化对氯喹抑制恶性疟原虫的效果差异显著,而乙胺嘧啶抑制恶性疟原虫的效果,受二氧化碳浓度的影响不大.结论进行恶性疟原虫培养和耐药性实验时应根据二氧化碳的使用浓度而选择适当的碳酸氢钠浓度.
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| 关 键 词： | 疟原虫,恶性 氯喹耐药性 二氧化碳 |
STANDARDISATION OF THE BICARBONATE BUFFER FORCULTIVATION OF PLASMODIUM FALCIPARUM AND EVALUATION OF DRUG RESISTANCE |
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| Abstract. STANDARDISATION OF THE BICARBONATE BUFFER FORCULTIVATION OF PLASMODIUM FALCIPARUM AND EVALUATION OF DRUG RESISTANCE[J]. Journal of Pathogen Biology, 2002, 15(1): 14-17 |
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| Authors: | Abstract |
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| Abstract: | Objective To investigate the differential effects of drugs on Plasmodium falciparum by changes in using bicarbonate buffer. Methods Effects of antimalarial drugs on parasite growth were assayed by monitoring uptake of hypoxanthine. Results The inhibitory effect of chloroquine, on P. falciparum (K1 strain) is affected by pH differences caused by various CO 2 concentrations in the presence of a constant NaHCO 3 concentration. In contrast, inhibition by pyrimethamine on the growth of the K1 strain was unaffected by different CO 2 concentrations. Conclusion Conventional medium for P. falciparum culture should be modified with constant NaHCO 3 according to the CO 2 concentration to evaluate drug resistance of P. falciparum . |
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| Keywords: | Plasmodium falciparum chloroquine resistance CO 2 |
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