| 左归丸与右归丸对EAE大鼠淋巴细胞亚群和NK细胞的影响 |
| |
| 引用本文： | 叶明,樊永平,王蕾,龚海洋,周莉,王雅杰. 左归丸与右归丸对EAE大鼠淋巴细胞亚群和NK细胞的影响[J]. 中华中医药杂志, 2009, 0(3) |
| |
| 作者姓名： | 叶明 樊永平 王蕾 龚海洋 周莉 王雅杰 |
| |
| 作者单位： | 首都医科大学附属北京天坛医院;首都医科大学中医药学院; |
| |
| 基金项目： | 北京市中医药科技发展基金项目(No.2003Ⅲ-21); 北京市中医药重点学科基金项目(No.2004京中重Ⅳ14); 北京市自然科学基金项目(No.7062022) |
| |
| 摘 要： | 目的:通过观察左归丸与右归丸对大鼠实验性自身免疫性脑脊髓炎模型(EAE)的影响,了解两者作用机制的异同。方法:应用髓鞘碱性蛋白与完全福氏佐剂按体积比1 1制成抗原并于Lewis大鼠双后足垫下注射,建立EAE模型。通过流式细胞仪技术观察淋巴细胞亚群及NK细胞水平。结果:造模后第15天,左归丸组CD4+细胞显著升高(P<0.05);模型组CD4+/CD8+比值高于正常组(P=0.052)。造模后第27天,模型组CD4+/CD8+比值明显升高(P<0.05);左归丸组CD4+/CD8+比值明显降低(P<0.05);左归丸组和右归丸组NK细胞明显升高(P<0.05)。结论:EAE大鼠CD4+/CD8+比值升高,这在EAE发病中发挥重要作用。滋阴补肾法可上调急性期CD4+细胞水平,下调缓解期CD4+/CD8+比值来治疗EAE。左归丸与右归丸可下调急性期NK细胞水平,上调缓解期NK细胞水平治疗EAE。
|
| 关 键 词： | 实验性自身免疫性脑脊髓炎 多发性硬化 中医药 淋巴细胞亚群 自然杀伤细胞 |
Effects of ZuoGuiWan and YouGuiWan on lymphocyte subsets and NK cells in peripheral blood of EAE rats |
| |
| YE Ming,FAN Yong-ping,WANG Lei,GONG Hai-yang,ZHOU Li,WANG Ya-jie. Effects of ZuoGuiWan and YouGuiWan on lymphocyte subsets and NK cells in peripheral blood of EAE rats[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2009, 0(3) |
| |
| Authors: | YE Ming FAN Yong-ping WANG Lei GONG Hai-yang ZHOU Li WANG Ya-jie |
| |
| Affiliation: | YE Ming1,FAN Yong-ping1,WANG Lei2,GONG Hai-yang1,ZHOU Li1,WANG Ya-jie1(1Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China,2School of Traditional Chinese Medicine,Beijing 100013,China) |
| |
| Abstract: | Objective: To observe the effect of ZuoGuiWan and YouGuiWan on induced experimental autoimmune encephalomyelitis(EAE) in Lewis rats,compare the therapeutic effect between ZuoGuiWan and YouGuiWan,and explore the underlying immunoregulatory mechanism between warming-yang and nourishing-yin therapy.Methods : 120 male Lewis rats were bred at Capital Medical University under specifi c pathogen-free conditions.24 rats were selected as normal group before immunization.The other rats were randomly devided into 4 gr... |
| |
| Keywords: | Exerimental autoimmune encephalomyelitis Multiple sclerosis Traditional Chinese medicine Lymphocyte subsets Natural killer cell |
| 本文献已被 CNKI 等数据库收录！ |
|
