Interleukin 1 induces specific phosphorylation of a 41 kDa plasma membrane protein from the human tumor cell line K 562 |
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| Authors: | M Martin D Lovett K Resch |
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| Affiliation: | 1. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva, Switzerland;2. School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva, Switzerland;3. Division of BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands;4. Department of Biomolecule Generation and Optimization, Institut de Recherche Pierre Fabre, Centre d''Immunologie Pierre Fabre, Saint-Julien-en-Genevois, France;5. Department of Anesthesiology, Pharmacology and Intensive Care, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland;1. IRCCS Humanitas Clinical and Research Center, via Manzoni 56, 20089 Rozzano Milan, Italy;2. Humanitas University, via Rita Levi Montalcini, 20090 Pieve Emanuele Milan, Italy;3. William Harvey Research Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK;4. Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA;5. Department of Medicine, Radboud University Medical Center, Nijmegen, the Netherlands;1. Novartis Institute for Biomedical Research, Novartis Pharmaceuticals Corporation, USA;2. Oncology Business Unit, Novartis Pharmaceuticals Corporation, Switzerland |
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| Abstract: | An in vitro system was established to investigate the early effects of interleukin 1 (IL 1) on a clearly defined subcellular system, the plasma membrane. Murine IL 1 was obtained from the macrophage tumor line P 388 D1 and purified to apparent homogeneity. Plasma membranes from the human leukaemic tumor cell line K 562 were highly purified by differential ultracentrifugation. The monokine induced the specific phosphorylation of a 41 kDa plasma membrane protein. This could be demonstrated by incubating the plasma membranes in the presence of IL 1 and (gamma-32P)-ATP, separating the proteins by SDS-PAGE and subsequent autoradiography. A first biochemical reaction caused by IL 1 in the target organelle of the cell, the plasma membrane, was discovered, yielding a clue of how signal transmission may take place finally resulting in the stimulation of the responding cell. |
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