增殖细胞核抗原和p27在鼻内翻性乳头状瘤中的表达

目的 检测鼻内翻性乳头状瘤(nasal inverted papilloma,NIP)组织中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)和p27蛋白表达,从细胞增殖状况和细胞周期调控方面探讨NIP发生、生长机制.方法 应用免疫组织化学LSAB法检测47例NIP、49例鼻息肉、43例鼻鳞状细胞癌、11例正常鼻黏膜组织中PCNA和p27蛋白的表达.结果 NIP组PCNA标记指数(labelling index,LI)高于鼻息肉组及正常鼻黏膜组(P＜0.001),与鼻鳞状细胞癌组差异无统计学意义(P＞0.05),NIP中初发组与复发组之间差异无统计学意义(P＞0.05);p27蛋白在各实验组中均有部分表达,各组间差异无统计学意义(P＞0.05).结论 PCNA指数可以反映NIP的增殖状况,但其与NIP的复发无明显关系;而p27在NlP发展过程中的作用尚需进一步探讨.

Expression and significance of PCNA and p27 in inverted papilloma of nasal cavities and paranasal sinuses

OBJECTIVE To investigate the expression of proliferating cell nuclear antigen (PCNA)and p27 protein in inverted papilloma (NIP)of nasal cavities and papransal sinuses and to explore the mechanism of the formation and growth of NIP from cell proliferating condition and cell cycle regulation. METHODS PCNA, p27 protein were stained with immunohistochemical technique (LSAB). There were four groups of patients47 cases in the NIP group, 49 cases in the nasal polyps group, 43 cases in the nasal squamous carcinoma group and 11 cases in the normal control group. RESULTS The PCNA labeling index(LI)in NIP group was significantly higher than that in the groups of nasal polyps and normal nasal mucosa (P<0.001). But no significant difference was found between NIP and nasal squamous carcinoma group(P>0.05). At the same time, there was no difference between primary NIP and recurrent NIP group (P>0.05). p27 protein was expressed in all groups, but no difference was found among them (P>0.05). CONCLUSION The PCNA labeling index may reflect the proliferating condition of NIP, but does not have relationship with NIP recurrence. And the role of p27 in the development of NIP needs more investigation.