桂林地区乙肝患者HBV基因型分布及S基因变异研究

目的研究桂林地区HBV基因型分布、S基因突变类型以及基因型与乙肝患者血清标志物、DNA载量和S基因突变位点的相关性。方法用巢式PCR方法扩增乙肝患者HBV的S基因,经测序后与标准序列比对,构建系统树进行基因分型,分析S基因的突变位点并通过对相关氨基酸的分析判断血清型,采用t检验和χ2检验统计分析相关性。结果 84例乙肝患者HBV基因型有B型(59.5%)、C型(40.5%)。B基因型中的血清型有adw1型(84%)、ayw1型(4%)、adrq+型(10%)。C基因型中有adrq+型(88.2%),adw1型(8.8%)。S基因突变位点主要为T126I和T143M/S,突变率分别达34.1%和40.0%。基因型与患者年龄及DNA载量具相关性(P<0.05),与乙肝患者血清标志物及S基因突变位点无相关性(P>0.05)。结论桂林地区HBV基因型主要为B型,其次为C型。血清型主要为adw1型和adrq+型。S基因变异点集中,有形成优势变异株的趋势。

HBV genotypes distribution and the S gene mutations among patients infected with HBV in Guilin

【Objective】 To investigate the distribution of HBV genotypes and the mutations of S gene in the Guilin and the association between genotype and the serum mark,DNA loads and the mutations of S gene.【Methods】 The S gene of hepatitis B virus was amplified by nest PCR and directly sequenced.The viral genotype was identified after phylogenetic analysis.The mutations of S gene were detected and the HBsAg subtype-specific amino acids were analyzed based on the amino acid sequences.The association between genotype and the serum mark,DNA loads and the mutations of S gene were analyzed using t test and χ2 test.【Results】 We found 2 genotypes in hepatitis B patients in Guilin: B(60.0%) and C(38.2%).In genotype B,the proportion of adw1 was 84%,ayw1was 4%,adrq+ was 10%;in genotype C,the proportion of adrq+was 88.2%,adw1 was 8.8%.The predominant mutations of S gene were T126I/A(34.1%) and T143M/S(40.0%).There was a correlation between HBV genotype and the age of patient,DNA loads(P <0.05),but no correlation between HBV genotype and the serum mark,mutations of S gene(P >0.05).【Conclusion】 Genotype B and C are predominant in Guilin;Adw1 and adrq+ are majority serotype;the high mutation sites of S gene were found,there is a tendency to form major variants.