| 结肠直肠肿瘤筛检的一种新方法：PNA—HRP法检测直肠粘液的T抗原 |
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| 引用本文： | 孙鹏 陈宗佑. 结肠直肠肿瘤筛检的一种新方法：PNA—HRP法检测直肠粘液的T抗原[J]. 外科理论与实践, 1998, 3(3): 143-145 |
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| 作者姓名： | 孙鹏 陈宗佑 |
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| 作者单位： | 上海医科大学附属华山医院普外科 200040(孙鹏，陈宗佑，余枫，邓景德)，上海医科大学附属华山医院普外科 200040(张延龄) |
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| 摘 要： | 建立一项简易的结肠直肠肿瘤的筛选检查方法。方法:采用亲合组织化学方法,用辣根过氧化酶(HRP)标记的花生凝集素(PNA)检测直肠粘液的T抗原。用肛指检查取得直肠粘液,涂于载玻片上。与HRP标记的PNA起反应,呈棕色者为阳性。结果:检测结肠直肠癌的敏感性为86.6％,特异性为92.5％。结论:直肠粘液T抗原检测筛选结肠直肠肿瘤是一种简易敏感的方法,较适合于人群普查。
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| 关 键 词： | T抗原 结肠直肠癌 花生凝集素 辣根过氧化酶 |
A New Screening Test of Colorectal Tumor: PNA-HRP Method for Detecting T Antigen in Rectal Mucus |
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| SunPeng,Chen Zongyou,Yu Feng,et al.. A New Screening Test of Colorectal Tumor: PNA-HRP Method for Detecting T Antigen in Rectal Mucus[J]. Journal of Surgery Concepts & Practice, 1998, 3(3): 143-145 |
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| Authors: | SunPeng Chen Zongyou Yu Feng et al. |
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| Abstract: | To develop a new easy test for screening examination of colorectal neoplasia. Methods: Mucus, obtained by digital rectal exam, is smeared on a slide to react with peanut agglutinin (PNA) labe-led by Horseradish-peroxidase(HRP). Results: Positive reaction shows a brown color. The sensitivity is 86.6%, and is specificity 92.5% for colorectal neoplasia. Conclusions: The rectum mucus slide test is sensi-tive for screening examination of colorectal neoplasia. |
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| Keywords: | T-Antigen PNA HRP Colorectal neoplasia |
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