汉族酒依赖高发家系单胺氧化酶A基因多态性的对照研究

目的 了解单胺氧化酶Ａ（ＭＡＯＡ）基因多态性在汉族酒依赖高发家系和对照家系中的分布特点及对酒哪病的影响。方法 采用美国酒依赖遗传研究协作组的半定式酒依赖遗传量表，按入组标准筛选出９个酒依赖高发家系４９名成年个体（其中有酒依赖患者２３例）及８个对家系４５名成年个体。采用多聚酶链式反应和限制性片段长度多态性ＥｃｏＲＶ酶切法检测ＭＡＯＡ基因。结果 酒依赖高发家系与对照家系间ＭＡＯＡ等位基因频率差异有非常

A control study on the polymorphism of the ADH and ALDH genes in high risk alcoholic families of Han ethnic population

Objective The purpose of this study was to investigate the characteristics of the polymorphism of ADH2, ADH3 and ALDH2 genes in the high risk alcoholic families of Han ethnic population Methods The 49 members (including 23 alcohol dependents) of 9 alcoholic families, each family consisting at least 2 alcohol dependent adults, and 45 members of 8 control families (without any alcoholics) were identified according to the SSAGA (Semi Structured Assessment for the Genetics of Alcoholism) from the COGA (The Collaborative Organization on the Genetics of Alcoholism) The genotypes and allele frequencies of ADH/ALDH genes were investigated by polymerase chain reaction (PCR) and allele specific oligonucleotide (ASO) hybridization Results All of the members of the alcoholic families were ALDH2*1 homozygous without any ALDH2*2 gene The significant difference of the allele frequency of ALDH2 was observed between the alcoholic and control families ( P < 0 01) The allele frequency of ADH2*1 was more frequent in alcoholic families than control There was no difference in the frequency of ADH3 allele between the alcoholic and control families Conclusions The allele of ALDH2*1 may be an important risk factor And the ADH2 gene may also play a role in high risk alcoholic families in Han ethnic population