| 54株分枝杆菌国际参考株16S rRNA序列分析 |
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| 引用本文： | 闫李侠,黄至澄,徐黔宁,陈保文,王国治. 54株分枝杆菌国际参考株16S rRNA序列分析[J]. 江西医学检验, 2011, 0(5): 469-471 |
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| 作者姓名： | 闫李侠 黄至澄 徐黔宁 陈保文 王国治 |
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| 作者单位： | 台州市第一人民医院;中国药品生物制品检定所; |
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| 基金项目： | 国家科技重大专项课题,编号:2009ZX10004-805 |
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| 摘 要： | 目的分析54株分枝杆菌国际参考株16S rRNA序列,为临床分离株的鉴定提供参考。方法用16S rRNA序列分析法对54株分枝杆菌国际参考株进行分析,构建系统发育树,计算相似性百分比。结果除11株（共9种4组）分枝杆菌,即产鼻疽分枝杆菌与塞内加尔分枝杆菌;溃疡分枝杆菌与海分枝杆菌;堪萨斯与胃分枝杆菌;3株结核分枝杆菌与田鼠分枝杆菌、非洲分枝杆菌16S rRNA基因序列完全相同无法相互鉴别,其它41株（共41种）分枝杆菌菌种间16S rRNA均不相同可以得到很好的鉴别。结论 16S rRNA基因序列分析是一种很好的鉴定分枝杆菌的方法,国际参考株16S rRNA基因序列的研究弥补了基因数据库的不足。
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| 关 键 词： | 分枝杆菌 16S rRNA 序列分析 |
Analysis of the genotype in 54 reference strains of Mycobacteria based on 16S rRNA gene sequences |
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| YAN Lixia,HUANG Zhicheng,XU Qianning,et al.. Analysis of the genotype in 54 reference strains of Mycobacteria based on 16S rRNA gene sequences[J]. Jiangxi Journal of Medical Laboratory Sciences, 2011, 0(5): 469-471 |
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| Authors: | YAN Lixia HUANG Zhicheng XU Qianning et al. |
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| Affiliation: | YAN Lixia,HUANG Zhicheng,XU Qianning,et al.Taizhou First People's Hospital,Zhejiang Taizhou 318020,China |
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| Abstract: | Objective To analyze the genotype in 54 reference strains of mycobacteria for the identification of clinical isolates.Methods The genotypes in 54 reference strains of mycobacteria were analyzed with 16S rRNA sequence,then phylogenetic tree was constructed and similarity was calculated.Results With the sequencing of 16S rRNA in the 54 reference strains of mycobacteria,all the mycobacteria could be discriminated except M.farcinogenes and M.senegalense;M.ulcerans and M.marinum;M.gastri and M.kansasii;3 strains... |
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| Keywords: | Mycobacteria 16S rRNA Reference strains |
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