HPLC法同时测定黄芩中黄芩苷、黄芩素、汉黄芩素的含量

目的:建立反相高效液相色谱法同时测定黄芩中黄芩苷、黄芩素、汉黄芩素的含量.方法:采用Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-0.1％磷酸溶液为流动相,梯度洗脱；检测波长为277 nm；流速1.0 ml·min -1；进样量10 μl;柱温35℃.结果:黄芩苷、黄芩素、汉黄芩素的线性范围分别为0.059 ～ 1.175 μg,r=1.000 0(n =7)、0.010～0.204μg,r=1.000 0(n =7)和8.210×10-3～1.642×10-1 μg,r =1.000 0(n =7)；提取回收率分别为99.08％,RSD=0.5％(n=6)、98.66％,RSD =0.5％ (n =6)和98.31％,RSD =0.7％ (n =6).结论:本法操作简便、快速、结果准确,可用于黄芩的质量控制.

Simultaneous Determination of Baicalin, Baicalein and Wogonin In Radix Scutellariae by HPLC

Objective： To establish a RP-HPLC method for the simultaneous determination of baicalin, baicalein and wogonin in Radix Scutellariae. Method： An Agilent Eclipse XDB-CIs （250 mm × 4. 6 mm,5 txm） column was used. The mobile phase was metha- nol-acetonitrile-0. 1% H3PO4 with gradient elution and the detection wavelength, was 277 nm. The flow rate was 1.0 ml min-1, the in- jection volume was 10 txl and the column temperature was 35℃. Result： The linear response of baicalin ranged from 0. 059-1. 175 ug （r = 1. 000 0,n =7） ,and that for baicalein and wogonin was 0. 010-0. 204 ug （r = 1. 000 0,n =7） and 8. 210 x 10-3-1. 642 x 10-1 ug （r = 1. 000 0,n = 7 ）, respectively. The average recovery of baicalin, baicalein and wogonin was 99.08% （ RSD = 0. 5% , n = 6 ）, 98.66% （RSD = 0. 5% ,n =6） and 98.31% （RSD =0. 7% ,n =6） ,respectively. Conclusion： The method is convenient,quick and accurate, and which can be used for the quality control of Radix Scutellariae.