实验观察依替米星和庆大霉素的肾脏毒性

目的探讨依替米星、庆大霉素致肾小管上皮细胞凋亡和病理学改变。方法雄性Wistar大鼠18只随机分均为庆大霉素组、依替米星组和溶媒对照组,分别腹腔注射庆大霉素、依替米星100mg.kg-1.d-1和生理盐水,连续给药5d。末次给药24h后,用高效液相色谱法(HPLC)、荧光偏振免疫分析法(FPIA)测定肾脏组织中庆大霉素、依替米星药物含量,TUNEL法观察肾脏细胞凋亡,HE染色观察肾脏组织病理学改变。结果庆大霉素组和依替米星组肾脏组织药物浓度分别为(487.9±97.3)μg/g和(463.4±120.2)μg/g,肾小管细胞凋亡数分别为(1112.0±353.1)个/mm2和(820.9±243.0)个/mm2(P>0.05)。与庆大霉素组相比,依替米星组的肾脏组织病理学改变轻。结论依替米星致肾脏毒性较庆大霉素小。

A experimental investigation on the nephrotoxicity induced by etimicin and gentamicin

Objective To investigate the nephrotoxicity induced by gentamicin and etimicin.Methods Eighteen male Wistar rats were equally randomized into three groups.Thr rats in groups of A and B were intraperitoneally injected gentamicin and etimicin 100 mg·kg-1·d-1 for 5 days,and those in group C were injected normal saline as the controls.All animals were sacrificed 24 h after the last dosing and both kidneys were harvested.The contents of gentamicin and etimicin were measured with HPLC and fluorescence polarization immunoassay(FPIA),respectively.The apoptosis of renal tubular epithelial cells was examined by TUNEL method.Morphologic changes of the kidney were examined by HE staining.Results The contents of gentamicin and etimicin in renal cortex were(487.9±97.3) μg/g and(463.4±120.2) μg/g,respectively.The number of apoptotic renal tubular epithelial cells for groups of A and B were(1112.0±353.1) pieces/mm2 and(820.9±243.0) pieces/mm2,respectively(P>0.05).The degree of pathological morphologic changes in group B was slighter than that in group A.Conclusion The degree of nephrotoxicity induced by etimicin is slighter than that induced by gentamicin.