| 基于UPLC-MS/MS的芍药苷及其代谢产物在大鼠体内药动学研究 |
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| 引用本文： | 霍晓光,王琨,冯心池,丁丽琴,邱峰.基于UPLC-MS/MS的芍药苷及其代谢产物在大鼠体内药动学研究[J].中草药,2017,48(17):3582-3586. |
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| 作者姓名： | 霍晓光 王琨 冯心池 丁丽琴 邱峰 |
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| 作者单位： | 天津中医药大学中药学院, 天津 300193;天津中医药大学中医药研究院, 天津 300193;天津市现代中药重点实验室, 天津 300193;天津中医药大学中药学院, 天津 300193;天津中医药大学中医药研究院, 天津 300193;天津市现代中药重点实验室, 天津 300193;天津中医药大学中药学院, 天津 300193;天津市现代中药重点实验室, 天津 300193;天津中医药大学中医药研究院, 天津 300193;天津市现代中药重点实验室, 天津 300193;天津中医药大学中药学院, 天津 300193;天津市现代中药重点实验室, 天津 300193 |
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| 基金项目： | 国家自然科学基金重点资助项目（81430095）；教育部博士点基金（20132134110006）；天津市应用基础及前沿技术研究计划（16JCYBJC27900） |
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| 摘 要： | 目的建立快速、灵敏、简便的超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定大鼠血浆中芍药苷及其代谢产物芍药内酯苷的暴露量,并研究单次ig给予大鼠低、中、高剂量(20、60、120 mg/kg)的芍药苷水溶液后,芍药苷、芍药内酯苷在大鼠体内的药动学特征。方法以栀子苷为内标,血浆样品经甲醇(含0.1%甲酸)沉淀蛋白后,通过ACQUITY UPLC BEH-C_(18)柱(50 mm×2.1 mm,1.7μm),以0.1%甲酸水溶液-乙腈为流动相梯度洗脱,色谱运行时间为4.5 min。采用电喷雾离子源(ESI),负离子扫描模式,以多反应监测方式(MRM)进行定量测定。结果芍药苷、芍药内酯苷的标准曲线的线性范围均为2.00~400 ng/m L,定量下限均为2.00 ng/m L,2者日内和日间精密度RSD均小于9.10%。芍药苷在低、中、高3个剂量给药组中的t_(max)分别为(1.56±0.62)、(1.13±0.35)、(1.28±1.92)h,Cmax分别为(85.45±47.49)、(390.75±139.26)、(1 223.5±420.15)μg/L;其中芍药苷水溶液低、中剂量组未检测到代谢产物芍药内酯苷,芍药苷水溶液高剂量组芍药内酯苷的tmax为(1.81±0.53)h,C_(max)为(19.81±8.98)μg/L。结论本方法简便、准确、专属性强,适用于芍药苷和芍药内酯苷在大鼠体内的药动学研究。
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| 关 键 词： | 芍药苷 芍药内酯苷 超高效液相色谱-串联质谱 药动学 代谢产物 |
| 收稿时间： | 2017/4/7 0:00:00 |
Determination of paeoniflorin and its metabolite in plasma of rats by liquid chromatography-tandem mass spectrometry |
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| HUO Xiao-guang,WANG Kun,FENG Xin-chi,DING Li-qin and QIU Feng.Determination of paeoniflorin and its metabolite in plasma of rats by liquid chromatography-tandem mass spectrometry[J].Chinese Traditional and Herbal Drugs,2017,48(17):3582-3586. |
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| Authors: | HUO Xiao-guang WANG Kun FENG Xin-chi DING Li-qin and QIU Feng |
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| Institution: | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | paeoniflorin albiflorin UPLC-MS/MS pharmacokinetics metabolite |
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