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不育患者血清精子蛋白17抗体的检测及临床意义
引用本文:张春华,李芳秋,杨爱龙,孙伟,缪家文. 不育患者血清精子蛋白17抗体的检测及临床意义[J]. 中华男科学杂志, 2007, 13(1): 27-29
作者姓名:张春华  李芳秋  杨爱龙  孙伟  缪家文
作者单位:1. 南京军区南京总医院解放军临床检验医学研究所,江苏,南京,210002;东南大学医学院江苏省基因诊断与治疗医学重点实验室,江苏,南京,210009
2. 南京军区南京总医院解放军临床检验医学研究所,江苏,南京,210002
基金项目:江苏省医学重点学科建设项目
摘    要:目的:检测抗精子抗体(AsAb)阳性不育患者血清中抗精子蛋白17(Sp17)的抗体,探讨该抗体在免疫性不育血清学诊断和免疫避孕方面的潜在应用价值。方法:用重组人Sp17作为抗原,ELISA法检测62例AsAb阳性血清中Sp17抗体阳性率及滴度,分析AsAb中抗Sp17的含量。结果:AsAb阳性血清中Sp17抗体阳性率为56.5%,男女之间差异无显著性。Sp17抗体占AsAb的(10.09±7.45)%,结果具有统计学意义(P<0.05)。结论:Sp17是重要的精子抗原组分,血清中Sp17抗体的检测可作为不育患者辅助诊断指标,同时也提示Sp17可能是一种免疫避孕候选抗原疫苗。

关 键 词:精子蛋白17  抗体  免疫性不育  免疫避孕
文章编号:1009-3591(2007)01-0027-03
收稿时间:2006-07-09
修稿时间:2006-10-25

Detection of Anti-Sp17 Antibodies in Infertile Patients'''' Serum and Its Clinical Significance
ZHANG Chun-hua,LI Fang-qiu,YANG Ai-long,SUN Wei,MIAO Jia-wen. Detection of Anti-Sp17 Antibodies in Infertile Patients'''' Serum and Its Clinical Significance[J]. National journal of andrology, 2007, 13(1): 27-29
Authors:ZHANG Chun-hua  LI Fang-qiu  YANG Ai-long  SUN Wei  MIAO Jia-wen
Affiliation:PLA Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. zch198028@163.com
Abstract:OBJECTIVE: To test anti-Sp17 antibodies in the serum of AsAb positive infertile patients, to investigate the proportion of anti-Spl7 antibodies in AsAb and their potential application to the serologic diagnosis of immune infertility and immunocontraception. METHODS: With human recombinant Sp17 as the antigen, the ELISA method was used to detect the positive rate, antibody titre and content of anti-Sp17 antibodies in the AsAb positive serum. RESULTS: The positive rate of anti-Sp17 antibodies in the AsAb positive serum was 56.5%, with no significant difference in the gender aspect. The percentage of anti-Sp17 antibodies in AsAb was (10.09 +/-7.45) %, with statistical significance (P <0.05). CONCLUSION: Sp17 is an important sperm antigen. Anti-Sp17 antibodies in the serum can be taken as auxiliary diagnostic index of infertility, and Sp17 is shown to be a potential candidate immunocontraception vaccine.
Keywords:sperm protein 17 antibody   immune infertility   immunocontraception
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