| 高效液相色谱法测定人血浆中西洛他唑的浓度 |
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| 引用本文： | 何菊英,张蓉,唐敏,夏培元,刘松青,代青.高效液相色谱法测定人血浆中西洛他唑的浓度[J].中国药房,2005,16(6):445-447. |
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| 作者姓名： | 何菊英 张蓉 唐敏 夏培元 刘松青 代青 |
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| 作者单位： | 1. 第三军医大学西南医院药学部,重庆市,400038
2. 第三军医大学新桥医院药学部,重庆市,400037 |
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| 摘 要： | 目的 :建立以高效液相色谱法测定人血浆中西洛他唑浓度的定量分析方法。方法 :色谱柱为迪马公司C18(150mm×4 6mm ,5μm ) ,流动相为乙腈 -水 (40∶60 ,V/V ) ,流速为1 4ml/min ,检测波长为254nm。结果 :西洛他唑检测浓度在25～2000ng/ml范围内线性关系良好 (r=0 9999),最低检测浓度为12 5ng/ml ,回收率为99 .99 %～101 .44 % ,日内、日间相对标准差分别为0 .20 %～2 90 %、0. 19 %～2. 13 % (n=5)。结论 :本方法简便、快捷、灵敏、准确 ,可用于西洛他唑人体药动学研究。
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| 关 键 词： | 西洛他唑 血药浓度 高效液相色谱法 |
| 文章编号： | 1001-0408(2005)06-0445-03 |
| 修稿时间： | 2004年5月9日 |
Determination of Cilostazol Concentration in Human Plasma by HPLC |
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| HE Juying,ZHANG Rong,TANG Min,XIA Peiyuan,LIU Songqing,DAI qing.Determination of Cilostazol Concentration in Human Plasma by HPLC[J].China Pharmacy,2005,16(6):445-447. |
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| Authors: | HE Juying ZHANG Rong TANG Min XIA Peiyuan LIU Songqing DAI qing |
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| Abstract: | OBJECTIVE: To propose a HPLC method for quantitative assessment of cilostazol in human plasma. METHODS: Chromatogram column was C18(150mm×4.6mm, 5μm), mobile phases were acetonitrile-water(40∶60,V/V), the flow rate was 1.4ml/min,determined wavelength was 254nm. RESULTS: Cilostazol concentration was in the linear range of 25～2000ng/ml(r=0.9 999)with the lowest determinable concentration of 12.5ng/ml,recoveries of cilostazol from plasma were 99.99%～101.44%,the relative standard variation of intra-day and inter-day were 0.20%～2.90% and 0.19%～2.13%(n=5) respectively. CONCLUSIONS: This method is s simple, convenient, accurate, and applicable for study of pharmacokinetics of cilostazol in clinic. |
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| Keywords: | Cilostazol Concentration in plasma HPLC |
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