Neuroprotective effects of infliximab in experimental spinal cord ischemic injury |
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| Authors: | Cagatay Guven Alp Ozgun Borcek Berker Cemil Gokhan Kurt Zuhal Yildirim Nese Lortlar Ucankus Nedret Kilic Necdet Ceviker |
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| Affiliation: | 1. Department of Neurosurgery, Gazi University, Faculty of Medicine, Be?evler, Ankara 06500, Turkey;2. Department of Neurosurgery, Faculty of Medicine, Fatih University, Ciftlik Caddesi No. 57, Emek, Ankara 06510, Turkey;3. Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Be?evler, Ankara 06500, Turkey;4. Department of Histology and Embryology, Faculty of Medicine, Gazi University, Be?evler, Ankara 06500, Turkey;2. Department of Biochemistry, Faculty of Medicine, University of Turgut Ozal, Ankara, Turkey;3. Department of Pathology, Faculty of Medicine, University of Turgut Ozal, Ankara, Turkey;1. Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul, Korea;3. Department of Thoracic Surgery, Seoul National University Bundang Hospital, Seoul, Korea;2. Department of Anesthesiology and Pain Medicine, SMU-SNU Boramae Medical Center, Seoul, Korea;1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic;2. Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic;3. Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic;4. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic;5. Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic;6. Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic;2. Department of Pathology, Hadassah–Hebrew University Medical Center, Jerusalem, Israel;3. Department of Orthopedic Surgery, Hadassah–Hebrew University Medical Center, Jerusalem, Israel. |
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| Abstract: | Reactive oxygen species (ROS) have been implicated in the pathogenesis of spinal cord injury after both ischemia–reperfusion (I/R) and trauma. This experimental study was designed to investigate the potential effects of infliximab, an anti-tumor necrosis factor-α agent, on I/R injury of the rabbit spinal cord. Eighteen New Zealand white rabbits were divided into three groups, each consisting of six rabbits: sham (no I/R), I/R, and infliximab (I/R + infliximab). Spinal cord ischemia was induced by applying an infrarenal aortic cross clamp for 30 minutes. At 48 hours after ischemia, animals were functionally evaluated using the Tarlov score. Changes in the spinal cord were observed by measuring tissue levels of malondialdehyde (MDA), glutathione (GSH), advanced oxidation protein products (AOPP), and superoxide dismutase (SOD) and by evaluating hematoxylin–eosin-stained sections. At 48 hours after ischemia, the Tarlov scores in the infliximab group were higher than those of the I/R group, MDA and AOPP levels in the I/R group were significantly higher than those in the sham and infliximab groups (p < 0.05), and SOD levels in the infliximab group were significantly higher than those in the I/R and sham groups (p < 0.05). The sham group had higher GSH levels than the infliximab group; however, the difference was not statistically significant (p > 0.05). Histological examination revealed that the infliximab group had significantly less vascular proliferation, edema, and neuron loss than the I/R group. These results indicate that infliximab may protect the spinal cord against injury in a rabbit I/R model. |
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