高压氧对急性一氧化碳中毒大鼠下丘脑-垂体-肾上腺轴的作用研究

目的:探讨高压氧(HBO)治疗对急性一氧化碳(CO)中毒大鼠下丘脑-垂体-肾上腺(HPA)轴的作用。方法:将36只大鼠按照随机数字表法分为正常对照组、CO中毒组和HBO治疗组各12只。建立急性CO中毒大鼠模型，给予HBO治疗7 d后，采用转录组测序(RNA-seq)进行各组大鼠脑组织基因检测，采用实时定量PCR(RT-...

Effects of hyperbaric oxygen on the hypothalamic-pituitary-adrenal axis in rats with acute carbon monoxide poisoning

Objective To investigate the effects of hyperbaric oxygen(HBO)on the hypothalamic-pituitary-adrenal(HPA)axis in rats with acute carbon monoxide(CO)poisoning.Methods Rat models with acute CO poisoning were established and treated with HBO for 7 days.RNA-sequencing(RNA-seq)was used to detect genes in the brain tissue of each group of rats,and real time quantitative PCR(RT-PCR)was adopted to analyze the pro-opiomelanocortin(POMC)of the genes that were differentiated by RNA-seq;the enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of rat serum adrenocorticotropic hormone(ACTH)and cortisol(COR);and immunohistochemistry(IHC)and western blot were applied to detect the expressions of POMC protein and ACTH protein receptor(ACTHR)in rats’brain.Results The results of RNA-seq and RT-PCR showed that 7 days after CO poisoning,the POMC gene expression in hypothalamus was significantly increased(P<0.05),and its expression was downregulated by HBO treatment(P<0.05).The results of IHC and western blot showed that,compared with the control group,POMC expressions in the cerebral cortex of the CO group were increased(P<0.05),and POMC expressions in the HBO group were decreased(P<0.05).ACTHR expressions were increased in the cerebral cortex of the CO group(P<0.05),but there was no significant difference between the HBO group and the CO group(P<0.05).The testing results showed that the levels of serum ACTH and COR in the CO group were significantly increased(P<0.05),and were decreased after HBO treatment(P<0.05).Conclusion Acute CO poisoning can activate the HPA axis through the POMC-ACTH-COR pathway,and HBO may exert a therapeutic effect by inhibiting the excessive activation of related genes,proteins,and hormones on the HPA axis.