Neuroprotective effect of mesna (2-mercaptoethane sulfonate) against spinal cord ischemia/reperfusion injury in rabbits |
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| Authors: | Habibullah Dolgun Zeki Sekerci Erhan Turkoglu Hayri Kertmen Erdal R. Yilmaz Murat Anlar Imge B. Erguder Hakan Tuna |
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| Affiliation: | 1. First Neurosurgery Clinic, Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey;2. Second Pathology Clinic, Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey;3. Department of Biochemistry, Ankara University Faculty of Medicine, Ankara, Turkey;4. Department of Neurosurgery, Ankara University Faculty of Medicine, Ankara, Turkey;1. Department of Neurosurgery, University of Health Sciences, Fatih Sultan Mehmet Education and Research Hospital, Istanbul, Turkey;2. Department of Physiology, Marmara University School of Medicine, Istanbul, Turkey;3. Department of Neurosurgery, University of Health Sciences, D??kap? Education and Research Hospital, Ankara, Turkey;4. Department of Biochemistry, Turkish Ministry of Health, Refik Saydam H?fz?s?hha Institute, Experimental Research and Application Center, Ankara, Turkey;5. Department of Anatomy, Hacettepe University School of Medicine, Ankara, Turkey;6. Department of Neurosurgery, Tarsus Medical Park, Mersin, Turkey;1. Ministry of Health Diskapi Yildirim Beyazit Training and Research Hospital, Neurosurgery Clinic, Ankara, Turkey;2. Ministry of Health Fatih Sultan Mehmet Training and Research Hospital, Neurosurgery Clinic, ?stanbul, Turkey;3. Department of Histology and Embryology, Ankara University Faculty of Medicine, Ankara, Turkey;4. Department of Biochemistry, Ankara University Faculty of Medicine, Ankara, Turkey;1. Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul, Korea;3. Department of Thoracic Surgery, Seoul National University Bundang Hospital, Seoul, Korea;2. Department of Anesthesiology and Pain Medicine, SMU-SNU Boramae Medical Center, Seoul, Korea |
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| Abstract: | Although the precise mechanism by which ischemia/reperfusion injury occurs in the spinal cord remains unclear, it is evident that free oxygen radicals and apoptosis play major roles in the destruction of membrane lipids, damage to DNA and cell death. The apoptotic process involves activation of the caspase-3 cascade. Although it is widely used as a protective agent against cell injury, it is unknown whether mesna (2-mercaptoethane sulfonate) ameliorates neuronal ischemic injury. The aim of this study was to determine the effect of mesna on caspase-3 activity in a rabbit model. Adult rabbits underwent spinal cord ischemic injury via occlusion of the abdominal aorta for 20 min. Twenty-four hours after ischemia, spinal cord samples were obtained and tissue caspase-3 activity was measured. Rabbits that had been given a single dose of 150 mg/kg mesna had decreased caspase-3 activity in the spinal cord following ischemia/reperfusion injury, indicating a protective effect. However, caspase-3 activity was lower in rabbits given methylprednisolone than in those given mesna, indicating that methylprednisolone has the stronger protective effect of the two agents. |
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