Germline mutation analysis of the androgen receptor gene in Finnish patients with prostate cancer |
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Authors: | Koivisto Pasi A Hyytinen Eija-R Matikainen Mika Tammela Teuvo L J Ikonen Tarja Schleutker Johanna |
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Affiliation: | Department of Clinical Genetics and laboratory of Cancer Genetics, University of Tampere, Tampere University Hospital, Finland. blpako@uta.fi |
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Abstract: | PURPOSE: Steroid hormones, particularly androgens, are suspected to have a major role in prostate carcinogenesis. Since androgen receptor mediates androgenic effects on cells and recent studies suggest that the androgen receptor gene is a putative prostate cancer susceptibility locus, we screened the coding region of the androgen receptor gene for germline mutations using the genomic DNA of patients with prostate cancer. MATERIALS AND METHODS: DNA samples from 38 patients with early onset prostate cancer and 36 from Finnish prostate cancer families showing no male-to-male transmission of prostate cancer were analyzed for mutations in the androgen receptor gene using single strand conformation polymorphism analysis and subsequent sequencing. RESULTS: R726L substitution in the hormone binding region of androgen receptor was found in 1 prostate cancer family but no previously uncharacterized germline mutations were detected. CONCLUSIONS: Our results indicate that constitutional androgen receptor mutations explain only a small fraction of familial and early onset prostate cancer cases in Finland. |
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