同种异体骨脱钙骨基质联合BMP修复兔桡骨骨缺损的实验研究

目的：探讨异体脱钙骨基质（DBM）骨泥复合骨形态发生蛋白（BMP）修复家兔桡骨节段性骨缺损的作用。方法：日本大耳白兔54只，雌雄不限，随机分为3组，A组：空白组（缺损区不植入任何材料），B组：对照组（缺损区植入金世植骨灵），C组：实验组（缺损区植入DBM骨泥）。建立家兔单侧桡骨骨缺损模型，采用同种异体骨DBM骨泥复合BMP进行修复，复合骨泥中加入骨胶原作为塑形剂，术后4、8、12周观察新骨生成情况，通过形态学、X线、组织学及计算机图像分析等观察指标，并与对照组及空白组进行比较，客观评价骨泥诱导成骨及骨缺损修复能力。结果：DBM与BMP复合骨泥易根据骨缺损大小塑形，术中操作简单。术后A组骨缺损未获骨性修复，B、C组骨缺损均获得骨性愈合，且新骨面积测定显示B、C组间成骨速度无明显差异（P〉0．05），比A组快（P〈O．01）。结论：异体DBM骨泥复合BMP具有良好的骨修复作用。

Experimental study of repairing radius defect with material combined bovine bone morphogenetic protein and allogenic demineralized bone matrix putty in rabbit

Objective： To discover the effect of material combined bovine bone morphogenetic protein （bBMP） and allogenic demineralized bone matrix （DBM） putty in repairing rabbit radius defect of fracture. Methods： 54 rabbits radius defect models were made and were divided into three groups in random. Group A with nothing,Group B with reconstituted bone xenograft （RBX, jinshizhiguling）, and Group C repared with the DBM and bBMP recombined putty. The new bone formation of each group were compared with others by observations, X-ray, histopathological analysis and computer-based image analysis, wich at the period in 4, 8, 12 weeks after operation , to evaluate the bone repairing effect. Results： The DBM putty could be shapen easily according to the defect in operation. Group A was nonunion after operation, complete bone union of the defect were achieved in Group B and Group C,and the measurement of new bone area demonstrated that bone formation speed had no significant differences between the groups of B and C （P〉0.05）,but significant difference compared to group A （P〈0.01）. Conclusion： Allogenic DBM putty combined bBMP have a good effect in repairing bone.