Viruses and renal carcinoma of Rana pipiens. VII. Propagation of a herpes-type frog virus |
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| Authors: | M Gravell A Granoff R W Darlington |
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| Affiliation: | 1. Aquaculture Vaccine Platform, Department of Microbiology, Faculty of Science, King Mongkut''s University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand;2. Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand;3. Department of Fisheries & Aquaculture, University of Agriculture Makurdi, P.M.B 2373 Makurdi, Nigeria;4. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand;1. ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata 700 120, West Bengal, India;2. ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, Dilkusha 226002, Lucknow, India;3. PMFGR Centre, ICAR-NBFGR, CMFRI Campus, Kochi 682 018, Kerala, India;4. Division of Fisheries, Indian Council of Agricultural Research, Krishi Anusandhan Bhawan - II, New Delhi 110 012, India;1. Department of Microbiology, Faculty of Science, King Mongkut''s University of Technology Thonburi (KMUTT), Bangkok, Thailand;2. Norwegian Veterinary Institute, Oslo, Norway;3. Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand;4. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand;5. Department of Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand |
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| Abstract: | A frog virus (FV 4) originally isolated by Rafferty (1965) from the urine of a frog bearing a Lucké renal adenocarcinoma has been cultivated and partially characterized. The virus multiplies at 25° with cytopathology in a Rana pipiens embryo cell line and can be plaque-assayed in these cells. Virus multiplication is relatively slow, and late in the growth cycle equivalent amounts of cell-associated and released virus are found. Infectivity is lost by exposure of virus to ether or to pH 3.0.Electron microscopic examination of infected cells reveals a nuclear site of virus synthesis. The virus size (95–100 mμ), morphology (icosahedral), and ultrastructure (162 capsomeres) are indistinguishable from those of the herpes-type virus seen in Lucké tumor cells and are similar to known herpesviruses. |
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