阿霉素上调肿瘤细胞Fas基因表达并诱导凋亡的实验研究

目的 研究化疗药物阿霉素(ADM)对肿瘤细胞Fas凋亡基因的调控，并探讨其致凋亡机制。方法 逆转录一聚合酶链反应(RT-PCR)比较经。ADM处理前后肿瘤细胞胃癌ADM细胞系SGG-7901、MGC-803和肺癌细胞系A-549的Fas mRNA的表达水平，同时观察其对抗Fas抗体的敏感性，并用流式细胞术检测肿瘤细胞凋亡率。结果 所有3种肿瘤细胞在ADM作用下Fas表达水平显著增强，差异有统计学意义(P＜0．05)，同时肿瘤细胞凋亡率明显增加(凋亡率分别为40．3％、43．6％、54．4％)。ADM能增加肿瘤细胞对Fas抗体的敏感性。结论 ADM上调肿瘤细胞Fas的表达，ADM与Fas抗体联合应用将增强肿瘤细胞的凋亡率，有一定的抗肿瘤临床应用前景。

The up-regulation of the expresion of Fas gene by adriamycin on tumor cells

Objective To investigate the modulation of anticancer drugs on the expression of Fas in tumor cells.Methods RT-PCR was used to detect the expression of Fas in tumor cells before and after co-incubated with adriamycin (ADM).Apoptosis of tumor cells was assayed by FCM.Results All the 3 tumor cell lines used in this study (gastric cancer cell lines SGC-7901,MGC-803,lung cancer cell line A-549 expressed Fas protein to varing degrees.ADM could enhance the expression of Fas gene in tumor cells (P<0.05),elevate the apoptosis rate ( 40.3 %, 43.6 %, 54.4 % respectively) and increase the susceptibility of them to anti-Fas antibodies.Conclusion ADM could up-regulate the expression of Fas in tumor cells.The combined use of ADM with Fas MoAb could sensitizes tumor cells to Fas-mediated apoptosis.