抑癌基因PTEN与pAkt在肝细胞肝癌中表达及其意义

目的研究肝细胞癌（HCC）中抑癌基因第10号染色体上缺失与张力蛋白同源的磷酸酯酶基因（PTEN）与磷酸化丝氨酸／苏氨酸蛋白激酶B（pAkt）表达及其意义。方法采用免疫组化S—P法检测65例HCC及癌旁组织中FFEN与pAkt表达并分析两者与肝癌患者年龄、临床分期、病理类型及侵袭转移性之间关系。结果HCC中PTEN蛋白阳性率明显低于癌旁肝组织，且随HCC恶性程度、侵袭转移性升高而显著下降（P〈0．05）；pAkt在HCC中表达率明显高于癌旁肝组织，且其表达率随HCC恶性程度、侵袭转移性升高而增加（P〈0．05）；肝癌及癌旁组织中，FFEN与pAkt表达均呈负相关（P〈0．01）。结论PTEN与pAkt表达与肝癌组织恶性程度相关，联合检测PTEN与pAkt在癌组织中表达情况，可作为肿瘤预后评估指标之一。

The expressions and clincal significance of PTEN and pAkt in hepatocellular carcinoma

Objective To investigate the expressions and clinical significance of the tumor supressor gene phosphatase and tensin homlog deleted on chromosome ten protein(PTEN) and oncogene phosphoserine/threonine protein kinase B(phospho-serine/threonine protein kinase Akt/PKB,pAkt) in hepatocellular carcinoma(HCC).Method The expressions of PTEN and pAkt in 65 cases of HCC and their pericarcinomatous tissues were detected by immunohistochemical staining with S-P method.Results The positive expression rate of PTEN in HCC was significantly lower than that in pericarcinomatous liver tissues,and the positive rate of pAkt was significantly higher than that in pericarcinomatous liver tissues(all P<0.01).With the progress of the tumor pathological differentiation,clinical stage and invasion,the positive rate of PTEN protein in HCC decreased;whereas that of pAkt increased(all P<0.05).The expression of PTEN was negatively associated with that of pAkt in HCC and pericarcinomatous liver tissues(all P<0.01).Conclusion The expessions of PTEN and pAkt may be associated with the progress of the tumor pathological differentiation.The detection of the expression of PTEN and pAkt may serve as as an index for prognosis of HCC.