PDE5基因沉默对肾透明细胞癌细胞增殖和凋亡的影响

目的 探讨PDE5基因对肾透明细胞癌细胞株786-O细胞增殖和凋亡的影响.方法 设计、合成PDE5 siRNA序列,按照HiPerFect转染试剂盒操作手册转染786-O细胞.采用实时定量聚合酶链反应(real-time quantitative PCR)和Western blot法检测转染后PDE5 基因的表达,通过WST-1法检测细胞增殖情况,caspase-3活性检测试剂盒检测caspase-3活性.结果 与空白对照组和阴性对照组相比较,PDE5 siRNA转染组的mRNA及蛋白表达水平显著降低,786-O细胞增殖能力明显受到抑制,caspase-3活性明显增加,差异均具有统计学意义(P<0.05).结论 siRNA干扰PDE5基因表达可抑制肾透明细胞癌细胞株786-O细胞的增殖并促进凋亡.

Effects of PDE5 Gene Silence on the Proliferation and Apoptosis of Clear Cell Renal Cell Carcinoma Cells.

Objective To explore the effect of PDE5 gene on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O. Methods The siRNA of PDE5 was constructed and transfected into 786-O cells with HiPerFect transfection reagent. The PDE5 expression levels after transfection were detected by using real-time quantitative PCR and Western blot. The cell proliferation was assessed by WST-1 assay. The caspase-3 activity was detected by kit. Results Compared with the control group and blank group, the mRNA and protein levels were significantly reduced and the proliferation of 786-O cells was significantly inhibited in the PDE5 siRNA group (P<0.05), while caspase-3 activity was significantly increased (P<0.05). Conclusion siRNA interference of PDE5 gene may inhibit cell proliferation and promote cell apoptosis in clear cell renal cell carcinoma cell line 786-O.