环氧交联去细胞牛颈静脉带瓣管道的血液相容性实验研究

目的评价环氧化合物（PC）交联去细胞牛颈静脉带瓣管道（BJVC）的血液相容性,探讨其在心血管外科和组织工程中的应用前景。方法选取本地健康杂种犬20只,采用随机数字表法分为实验组（n=10）和对照组（n=10）。实验组用PC交联的去细胞BJVC,对照组采用戊二醛（GA）交联的BJVC。运用兔血进行体外溶血实验计算溶血率,绘制时间-吸光度曲线;采用人全血测定D-二聚体和补体C3a des Arg水平评价其体外血液相容性。采用PC和GA制备的BJVC重建犬右心室-肺动脉连接,两组犬饲养10个月后,观察有无血栓形成评价其体内血液相容性。结果实验组溶血率（0.23%）低于对照组（0.35%）,符合医用生物材料的国家标准（〈5%）。实验组的凝血时间曲线较对照组平缓。实验组D-二聚体水平低于对照组,两组差异有统计学意义（0.10±0.01μg/ml vs.0.12±0.02μg/ml,t=3.277,P=0.004）,但均在正常范围内。实验组补体C3a des Arg水平低于对照组,两组差异有统计学意义（0.74±0.09μg/ml vs.1.02±0.19μg/ml,t=4.183,P=0.001）。犬右心室-肺动脉连接重建术后10个月每组各有8只生存,各有2只死于心室颤动。对照组3例有血栓形成,实验组无血栓形成。结论与GA交联相比,PC交联的BJVC体外、体内血液相容性均具优势,临床应用前景良好。

Research on Hemocompatibility of the Acellular Valved Bovine Jugular Vein Conduit Treated with Polyepoxy Compound

Objective To investigate the hemocompatibility of the acellular valved bovine jugular vein conduit(BJVC) treated with polyepoxy compound(PC),and discuss its application in cardiovascular surgery and tissue engineering in the future. Methods BJVC treated with PC was regarded as the experiment group and BJVC treated with glutaraldehyde(GA) was considered to be the control group.Rat blood was used for in vitro hemolytic test to calculate hemolytic rates of BJVC,and curve of absorbance-clotting time was drawn.H...