| 简并引物在扩增GBV-C/HGV NS3高度变异区的应用 |
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| 引用本文： | 王佑春,庄辉,R Ling.简并引物在扩增GBV-C/HGV NS3高度变异区的应用[J].中华微生物学和免疫学杂志,1998,18(5):417-420. |
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| 作者姓名： | 王佑春 庄辉 R Ling |
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| 作者单位： | 中国药品生物制品检定所，北京医科大学微生物系 |
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| 基金项目： | 英国WelcomeTrust基金 |
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| 摘 要： | 目的试图比较简并引物扩增ＧＢＶ－Ｃ／ＨＧＶＮＳ３变异较大的区域的作用。方法设计了简并引物和减温（ｔｏｕｃｈｄｏｗｎ）ＰＣＲ扩增程序以对目的基因进行扩增，并对阳性ＰＣＲ产物进行克隆及测序。结果该方法能够检测基因变异约达２１％的区域。结论提示该法是一项有效的了解基因家系的技术。
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| 关 键 词： | DNA引物 聚合酶链反应 肝炎病毒.庚型 |
Application of degenerate primers in amplifying highly variable regions of NS3 in GBV C/HGV |
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| R Ling.Application of degenerate primers in amplifying highly variable regions of NS3 in GBV C/HGV[J].Chinese Journal of Microbiology and Immunology,1998,18(5):417-420. |
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| Authors: | R Ling |
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| Abstract: | Objective To investigate the application of degenerate primers in amplifying highly variable regions of NS3 in GBV C/HGV. Methods The degenerate primers were used to amplify the highly variable regions in NS3 of GBV C/HGV with touchdown PCR program. The positive PCR products were cloned and sequenced. Results Up to 21% deversity regions of the virus were amplified by this method. Conclusion The degenerate primers can be used for amplification of highly variable regions or for indentification of uncharacterized viruses related to known virus groups. |
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| Keywords: | Degenerate primers Hepatitis G virus Polymerase chain reaction |
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