防己黄芪汤加减干预对阿霉素肾病大鼠肾组织MCP-1表达的影响

目的：观察探讨单核细胞趋化蛋白-1（MCP-1）在阿霉素肾病（AIN）大鼠的作用机制及防己黄芪汤加减对其表达的影响。方法：SD雄性大鼠随机分为4组,分别为正常组、模型组、防己黄芪汤＋雷公藤组（A组）及防己黄芪汤-甘草＋雷公藤组（B组）。造模予阿霉素（ADR）6.5mg/kg一次性尾静脉注射,正常对照予等量生理盐水尾静脉注射,注射后2周24h尿蛋白〉100mg为肾病模型成立。治疗A、B组分别予防己黄芪汤＋雷公藤及防己黄芪汤-甘草＋雷公藤灌胃,正常组与模型组予等量生理盐水灌胃。于第1、2、8周测24h尿蛋白定量,实验4、8周时留血,取肾,检测血生化,观察肾组织病理改变,测肾组织中MCP-1表达水平。结果：1）素造模后2周,各造模组24h尿蛋白定量均高于正常对照组（P〈0.05）,说明阿霉素单次尾静脉注射6.5mg/kg可成功制备MCNS模型;2）免疫组化和RT-PCR检测MCP-1表达情况,模型组与正常对照组比较MCP-1明显上调,两个中药治疗组与模型对照组比较MCP-1表达下调,A、B组之间比较MCP-1表达差异无统计学意义。结论：1）阿霉素肾病大鼠存在MCP-1mRNA转录和蛋白水平的异常,MCP-1表达的增加可能是阿霉素肾病蛋白尿产生的重要因素之一;2）本研究中去除组方中的甘草对防己黄芪汤加减疗效无明显影响;3）临床上应用防己黄芪汤加减治疗慢性肾病可能是通过降低蛋白尿、改善肾病理、抑制MCP-1的表达而发挥肾保护作用。

Experimental Study on Effects of Addition and Subtraction of Fangjihuangqi Decoction on the Expressions of MCP-1 mRNA and Protein in Renal Cortext of Adriamycin-induced Nephropathic Rats

Objective:To investigate mechanism of action of MCP-1 in minimal lesion nephrotic syndrome and effects of addition an subtraction of Fangjihuangqi decoction on the expressions of MCP-1 mRNA and protein in renal cortext of adriamycin-induced nephropathic rats.Methods:The rats were randomly divided into four groups(normal control group, nephropathy control group, Fangjihuangqi decoction adding Leigongteng group, Fangjihuangqi decoction adding Leigongteng and substracting Gancao group). ADR(6.5 mg/kg) was intr...