分割放疗对鼻咽癌组织细胞增殖凋亡的影响

目的：探讨常规分割放疗和超分割放疗对鼻咽癌组织细胞增殖凋亡的影响。方法：采用ＴｄＴ酶介导的生物素化ｄｕｔｐ缺口末端标记技术（ＴＵＮＥＬ）和免疫组织化学Ｓ－Ｐ法，分别检测６０例鼻咽癌患者在放疗第２周和第４周时细胞凋亡率（ａｐｏｐｔｏｓｉｓ ｒａｔｅ，ＡＲ）和增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｏｎ ｃｅｌｌ ｎｈｕｃｌｅａｒ ａｎｔｉｇｅｎ，ＰＣＮＡ）的表达。结果：常规分割放疗组放疗第４周后的ＡＲ

Effect of Conventional and Hyperfraction Radiotherapy on Apoptosis and Proliferation in Nasopharyngeal Carcinoma

Objective To investigate the effect of conventional and hyperfraction radiotherapy on apoptosis and proliferation in nasopharyngeal carcinoma(NPC).Methods Sixty cases of NPC were divided into two groups(conventional radiotherapy group and hyperfractionation radiotherapy group).Paraffin-embedded tissue blocks were obtained at the second and the fourth week of radiotherapy.The apoptosis rate(AR) and the expression of proliferation cell nuclear antigen(PCNA) were examined by terminal oxynucleotidyl transferase mediated dUTP biotin nick end labeling(TUNEL) and immunohistochemical s-p staining.Results The AR in the second week of conventional radiotherapy was higher and the expression of PCNA was lower compared with that in the fourth week(P<0.01);but there was no difference between the second and the fourth week in hyperfractionation radiotherapy group(P>0.05).Conclusion The decrease of apoptosis and the increase of proliferation in nasopharyngeal carcinoma during conventional radiotherapy shows that it is necessary to increase dose and fractions radiotion.