鼻咽癌组织中bcl-2基因断裂和bcl-2蛋白表达的检测及意义

为探讨ｂｌｃ－２基因与鼻咽癌发生的关系，采用分子原位杂交和免疫组化技术，检测４１例鼻咽癌组织中ｂｃｌ－２基因的两个断裂热点和蛋白表达。结果：４１例鼻咽癌发生ｂｃｌ－２基因表裂者４例，阳性率为９．８％，３４例出现ｂｃｌ－２蛋白阳性表达，阳性率为８２．９％；鼻咽良性病变阴性。ｂｃｌ－２基因断裂和蛋白表达与鼻咽癌组织学分级间无明显关系；ｂｃｌ－２蛋白表达与ｂｃｌ－２基因断裂间无相对应关系。提示：ｂｃｌ－

Significance of bcl-2 gene breaking and bcl-2 protein expression in nasopharyngeal carcinoma

In order to study the relationship between bcl-2 gene and nasopharyngeal carcinoma. The two hot breakpoints of bcl-2 gene and bcl-2 protein expression were examined by in situ hybridization and immunohistochemistry technique in 41 nasopharyngeal carcinoma (NPC ) tissues.bcl-2 gene breakpoint were found in 4 cases of NPC (positive rate 9. 8%). 34 cases of NPC expressed bcl-2 protein,the positive incidence was 82. 9%. No statistically significant differences was found in histological grades. All benign lesions were negative for bcl-2 gene breaking and bcl-2 protein expression. There was no corresponding relation between bcl-2 protein expression and bcl-2gene breaking. The results implicated that bcl-2 gene breaking may not paly an important role in the pathogenesis of NPC. It also suggested that overexpression of bcl-2 protein may involved in carcinogenesis of nasopharyngeal epithelium.