高肺血流对大鼠肺动脉结缔组织生长因子表达的影响

目的 探讨高肺血流对大鼠肺动脉结缔组织生长因子(connective tissue growth factor,CTGF) 表达的影响.方法 雄性SD大鼠32只,随机分为分流组(n=16)和对照组(n=16),对分流组大鼠行腹主动脉-下腔静脉穿刺术建立高肺血流动物模型.分别在分流术后4周、11周2个时间点处死大鼠(每组8只)留取肺组织标本.制作肺组织石蜡切片,观察肺动脉相对中膜厚度(relative median thickness,RMT)的改变;以免疫组织化学方法检测肺动脉胶原Ⅰ及CTGF表达的变化.结果 分流术后11周,分流组大鼠肺动脉RMT较对照组明显增加(P＜0.05);分流后4周、11周大鼠肺动脉胶原Ⅰ及CTGF表达较对照组明显增强(P均＜0.05).结论 高肺血流上调大鼠肺动脉CTGF表达,该作用可能参与介导高肺血流所致肺血管结构重建的机制.

Impact of high pulmonary blood flow on connective tissue growth factor expression in pulmonary artery of rats

Objectives To explore impact of high pulmonary blood flow on connective tissue growth factor (CTGF)expressed in pulmonary artery of rats. Methods Thirty-two(32)male SD rats were randomly divided into shunt group(n = 16)and control group(n = 16). Rats in the shunt group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of high pulmonary blood flow. In the control group,rats experienced the sham operation. Four weeks and 11 weeks after the operation,the changes of relative median thickness(RMT)of pulmonary artery were examined under optical microscope. The CTGF protein and collagen I protein expression in rat pulmonary artery were investigated by immunohistochemistry. Results Eleven(11)weeks after shunt operation,the RMT of pulmonary artery of shunt group increased significantly as compared with that of the control group(P < 0.05);Four weeks and eleven weeks after the operation,both expression of CTGF and collagen I in pulmonary artery of the shunt group were more up-regulated than those of the control group(P < 0.05). Conclusions High pulmonary blood flow up-regulated the pulmonary artery CTGF protein expression in rats,which might be involved in the mechanism of pulmonary vascular structure remodeling induced by high pulmonary blood flow.