基于iTRAQ标记结合生物信息学技术的风湿性疾病湿热证血清蛋白组学研究

目的 应用iTRAQ标记结合生物信息学分析技术，开展风湿性疾病湿热证的血清蛋白组学研究。方法 对强直性脊柱炎湿热证、痛风湿热证、正常对照共3组各20例血清，进行iTRAQ标记结合液相串联质谱蛋白组学分析，应用DAVID、UniProtKB/Swiss-Prot、IPA等网络数据库平台对风湿性疾病湿热证的差异蛋白进行生物信息学分析，并对显著差异蛋白进行验证。结果 获得15个湿热证差异蛋白，其中表达上调的蛋白12种，下调蛋白3种。通过分析得到差异表达蛋白所涉及的5个最有意义的典型生物信号通路。这些差异蛋白功能及生物信息学分析提示与疾病急性期反应和疾病活动度有关。验证实验结果与iTRAQ检测结果一致。结论 为风湿性疾病湿热证的分子机制以及候选诊断治疗标志物的进一步研究提供了依据。 

Study on Serum Proteomics of Rheumatism with Damp-heat Syndrome Based on iTRAQ Labeling Method Combined with Bioinformatics Technology

OBJECTIVE To investigate the serum proteomics of rheumatism with damp-heat syndrome by using iTRAQ labeling method together with bioinformatics technology. METHODS 20 serum samples respectively from ankylosing spondylitis with damp-heat syndrome group， arthrolithiasis with damp-heat syndrome group and normal control group were collected for proteomics analysis through iTRAQ labeling and liquid chromatography. Such network database platforms as DAVID， UniProtKB/Swiss Prot and IPA were resorted to perform bioinformatics analysis of differential protein in rheumatic diseases with damp-heat syndromes and verification would be done to the significant differential proteins. RESULTS Fifteen differential proteins of dampness-heat syndrome were obtained， with twelve being the up-regulated expression proteins and three being the down-regulated expression proteins. The five typical biological signaling pathways of most significant meaning related to differential protein expression were obtained through analysis. These differential protein functions and bioinformatic analysis were proved to be related to the acute phase responses and activities of diseases. Verification test results were consistent with those of iTRAQ. CONCLUSION This provides evidences for further study on molecular mechanism of rheumatic diseases with damp-heat syndromes as well as the candidate diagnostic markers.