菖蒲郁金汤对高皮质激素血症小鼠海马NF-L及SYP蛋白表达的影响

目的 探讨菖蒲郁金汤拮抗皮质酮诱导海马神经元损伤的作用。方法 将C57BL/6N小鼠随机分为空白组、模型组、氟西汀组、菖蒲郁金汤高剂量组、菖蒲郁金汤中剂量组及菖蒲郁金汤低剂量组。除空白组外，其余各组通过皮质酮诱导高皮质激素血症，予受试药物连续14d，观察小鼠体质量、脾脏质量及胸腺质量变化，采用强迫游泳实验和强迫悬尾实验评价菖蒲郁金汤对小鼠不动时间的影响，Western blot法检测神经微丝轻链蛋白（NF-L）及突触囊泡蛋白（SYP）蛋白表达。结果 菖蒲郁金汤可缓减模型动物体质量、脾脏质量及胸腺质量的降低（P<0.05）；减少强迫游泳实验、强迫悬尾实验小鼠不动时间（P<0.01）；上调NF-L及SYP蛋白的表达（P<0.01）。结论 菖蒲郁金汤通过上调NF-L及SYP的表达，拮抗高皮质激素血症所致的海马神经元损伤，发挥抗抑郁样行为效应。 

Effect of Acorus and Curcuma Decoction on Cortical Hormone in Mice Hippocampus NF-L and SYP Protein

OBJECTIVE To investigate the reverse effect of Acorus and Curcuma Decoction on despair like behavior in mice induced by hypercortisolism. METHODS 60 C57BL/6N mice were randomly divided into 6 groups: normal control， model control， Fluoxetine hydrochloride group， Acorus and Curcuma Decoction groups of high dose， middle dose and low dose. The hypercortisolism mice models were developed by subcutaneous corticosterone injection. Four weeks later， the changes of mice body， spleen and thymus mice weight were detected. After the forced swimming test and the tail suspension test finished， the mice were sacrificed by decapitation and the hippocampal tissues were rapidly collected for Western blot analysis of neurofilament light chain protein(NF-L) and synaptic vesicle protein(SYP). RESULTS Compared with the normal control group， the body weight was lower than model control group(P<0.05)， the immobility time was significantly prolonged in the forced swimming test and the tail suspension test (P<0.01)， spleen and thymus weight significantly decreased (P<0.05)， the expression of NF-L and SYP， were down-regulated in model control group(P<0.01). However， Acorus and Curcuma Decoction group， significantly shortened immobility time in behavioral science(P<0.01)， and up-regulated the expression of NF-L and SYP (P<0.01). CONCLUSION Acorus and Curcuma Decoction can protect neurons and antagonized the despair like behavior induced by hypercortisolism.