| 肠道菌群对汉黄芩苷的代谢研究 |
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| 引用本文： | 崔祥,陶金华,魏晓燕,江曙,徐君,钱大玮,段金廒.肠道菌群对汉黄芩苷的代谢研究[J].中草药,2017,48(19):4024-4028. |
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| 作者姓名： | 崔祥 陶金华 魏晓燕 江曙 徐君 钱大玮 段金廒 |
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| 作者单位： | 南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023,南京中医药大学 江苏省中药资源产业化过程协同创新中心, 江苏 南京 210023 |
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| 基金项目： | 国家自然科学基金资助项目（81673831） |
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| 摘 要： | 目的研究人肠道菌群对汉黄芩苷的代谢作用。方法采用UPLC-Q-TOF/MS技术分析肠道菌群对汉黄芩苷的代谢物;此外,采用16 S r DNA分子生物学方法进行肠道细菌的鉴定与组成分析。结果汉黄芩苷主要被代谢为汉黄芩素和少量的脱甲基代谢物,且肠道混合菌群的代谢能力较强。多数Enterococcus属和Escherichia属的肠道细菌具有代谢功能,其中具有较强代谢活性的一种单菌为Escherichia sp.41。结论汉黄芩苷主要被代谢为汉黄芩素和少量的脱甲基代谢物,鉴定出一种单菌为Escherichias sp.41。
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| 关 键 词： | 肠道细菌 汉黄芩苷 UPLC-Q-TOF/MS 代谢物 16 S rDNA |
| 收稿时间： | 2017/3/15 0:00:00 |
UPLC-Q-TOF/MS-based metabolic profiles of wogonoside by intestinal bacteria |
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| CUI Xiang,TAO Jin-hu,WEI Xiao-yan,JIANG Shu,XU Jun,QIAN Da-wei and DUAN Jin-ao.UPLC-Q-TOF/MS-based metabolic profiles of wogonoside by intestinal bacteria[J].Chinese Traditional and Herbal Drugs,2017,48(19):4024-4028. |
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| Authors: | CUI Xiang TAO Jin-hu WEI Xiao-yan JIANG Shu XU Jun QIAN Da-wei and DUAN Jin-ao |
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| Institution: | Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| Abstract: | Objective To investigate the metabolic effects of wogonoside by human gut microbiota. Methods UPLC-Q-TOF/MS was used to analyze the metabolites of wogonoside. Additionally, 16 S rDNA molecular biology method was applied to the identification and composition analysis of intestinal bacteria. Results Wogonoside was mainly metabolized to wogonin and small quantities of demethylated metabolites. Most of Enterococcus and Escherchia had the metabolic abilities, among which a single bacterium Escherichia sp. 41 possessed the stronger metabolic activity. Conclusion This study has a good guiding significance for in vivo process and efficacy of wogonoside. |
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| Keywords: | gut microbiota wogonoside UPLC-Q-TOF/MS metabolites 16 S rDNA |
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