丹参饮对冠脉结扎大鼠心肌缺血的保护研究

目的评价丹参饮对冠脉结扎大鼠心肌缺血的保护作用,并探讨其作用机制。方法雄性Wistar大鼠按体质量随机分为对照组、模型组、硝苯地平组、复方丹参片组和丹参饮低、中、高剂量组,每组各10只。硝苯地平剂量为0.167 mg/kg、复方丹参片剂量为10 g/kg,按10 m L/kg ig给药;丹参饮按0.5、1.0、2.0 m L/kg(相当于原药材0.27、0.54、1.08 g/kg)ig给药。各组动物均连续给药7 d。于第7天给药30 min后通过结扎冠脉建立大鼠心肌缺血模型。分别记录正常和结扎后心电图S-T段变化,测定血清乳酸脱氢酶(LDH)、肌酸激酶(CK)活性,计算心脏指数、左心室指数、左心室/心脏质量比和心肌梗死率。结果与模型组各时间点比较,丹参饮组在结扎后15~180 min心电图S-T段均明显降低,差异具有统计学意义(P0.05)。与模型组比较,各组LDH、CK活性均明显降低,差异具有统计学意义(P0.05);各组大鼠心脏指数、左心室指数、左心室/心脏质量比均明显降低,差异具有统计学意义(P0.05);各组心肌梗死质量和梗死率均明显降低,差异具有统计学意义(P0.05)。结论丹参饮对冠脉结扎大鼠心肌缺血具有保护作用,可能是增强冠脉供血和心肌代谢,改善心功能,有效抑制大鼠心肌缺血梗死。

Protection of Danshen Drink on myocardial ischemia in rats with coronary artery ligation

Objective To evaluate the protective effect of Danshen Drink on myocardial ischemia in rats with coronary artery ligation, and study its mechanisms. Methods Sixty male Wistar rats were randomly divided into control group, model group, nifedipine group, Compound Danshen Tablets group, and Danshen Drink low, medium, and high dose group according to body weight, and each group had 10 rats. The dose of nifedipine was 0.167 mg/kg, the dose of Compound Danshen Tablets was 10 g/kg, and rats in these group were ig administer 10 mL/kg. Rats in Danshen Drink groups were ig administer Danshen Drink 0.5, 1.0, and 2.0 mL/kg (Equivalent to 0.27, 0.54, and 1.08 g/kg of raw materials). All the animals were treated for 7 d. Myocardial ischemia model of rats was established by ligating coronary artery after 7th day of administration of 30 min. The ST segment changes of ECG after normal and ligation were recorded, the activity of serum lactate dehydrogenase (LDH) and creatine kinase (CK) were determined, and cardiac index, left ventricular index, left ventricular/heart mass ratio and myocardial infarction were calculated. Results Compared with the time points of the model group, the ST segments of the 15-180 min electrocardiogram in the Danshen Drink group were significantly decreased after the ligation, and the difference was statistically significant (P<0.05). Compared with the model group, LDH and CK activity in each group was significantly decreased, and there was difference between two groups (P<0.05); the rats cardiac index, left ventricular index, left ventricular/heart mass ratio were significantly decreased, and there was difference between two groups (P<0.05); myocardial infarction quality and infarct rate were significantly decreased, and there was difference between two groups (P<0.05). Conclusion Danshen Drink has protective effect on myocardial ischemia in rats with coronary artery ligation, which may enhance coronary blood supply and myocardial metabolism, improve cardiac function, and effectively inhibit myocardial ischemia in rats.